PXD072594

PXD072594 is an original dataset announced via ProteomeXchange.

Title	Nanostructures regulate cell adhesion behavior through mechanotransduction-mediated signaling pathways
Description	Nanotopography has a significant regulatory effect on cell adhesion behavior. We fabricated titanium alloy surfaces with titanium dioxide nanotubes using electrochemical anodization, which exhibited increased surface roughness and superhydrophilicity. Using single-cell force spectroscopy, we observed that the nanotubular structure altered the mechanical forces experienced at the cell-surface interface. Proteomic analysis revealed that Nesprin-1 protein transmits these signals to the intracellular core signaling system, leading to the upregulation and activation of GSK3β, STAT3, and related pathways. These events regulate cytoskeletal remodeling and adhesion complex assembly, thereby promoting cell adhesion.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_16:22:59.424.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shawn Shawn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-02 22:13:50	ID requested
⏵ 1	2026-05-24 16:23:00	announced

10.3390/jfb17040200;

Xiong C, Feng H, Lu L, Jing Z, Wang Y, Yang Y, Meng D, Zhang Y, Li W, Cai H, Nanotubes. J Funct Biomater, 17(4):(2026) [pubmed]

submitter keyword: mechanotransduction, mechanisms,TiO₂ nanotubes, single-cell force spectroscopy, cell adhesion

Chenao Xiong
contact affiliation	Department of Orthopaedics, Peking University Third Hospital
contact email	xiongca@pku.edu.cn
lab head
Shawn Shawn
contact affiliation	Department of Orthopaedics, Peking University Third Hospital
contact email	xiongca@pku.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD072594

PRIDE project URI

• PRIDE
  1. PXD072594
     1. Label: PRIDE project
     2. Name: Nanostructures regulate cell adhesion behavior through mechanotransduction-mediated signaling pathways