PXD072591

PXD072591 is an original dataset announced via ProteomeXchange.

Title	Proteomics for extracellular vesicles from cultured tanycytes with target knockdown versus control
Description	This work was designed to profile major proteins of extracellular vesicles (EVs) from mouse tanycytes with target gene knockdown (KD) versus control. The assay was based on using in vitro culture models of mouse hypothalamic tanycytes (predominantly alpha-tanycytes) which were engineered through lentivirus-mediated KD of a target gene, including CRISPR/Cas9-based Rab27a KD or Rictor KD or shRNA-based KD of pre-pro-insulin (ppIns). Scramble gRNA-based control was used as the control group for both Rab27a KD and Rictor KD, and scramble shRNA-based control was used as the control group for ppIns KD. EVs were released from these tanycytes in culture, purified and subjected to proteomics. EV samples of KD groups included Rab27a KD EVs (251015-1, 251015-2, 251015-5), ppIns KD EVs (251015-12, 251015-13, 251015-15), and Rictor KD EVs (251015-3, 251015-6, 251015-14). EV samples of control groups included scramble gRNA control EVs (251015-4, 251015-10, 251015-11) and scramble shRNA control EVs (251015-7, 251015-8, 251015-9). Proteomic profiling suggested that the overall protein composition of tanycyte-derived EVs was not substantially changed by either of these KD approaches.
HostingRepository	PRIDE
AnnounceDate	2026-02-16
AnnouncementXML	Submission_2026-02-16_15:07:45.303.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuna Choi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-02 17:19:14	ID requested
⏵ 1	2026-02-16 15:07:45	announced

10.1038/S42255-026-01474-3;

submitter keyword: None

Dongsheng Cai
contact affiliation	Department of Molecular Pharmacology, Einstein Institute of Neuroimmunology and Inflammation, Albert Einstein College of Medicine, Bronx, NY 10461, USA
contact email	dongsheng.cai@einsteinmed.edu
lab head
Yuna Choi
contact affiliation	Albert einstein college of medicine
contact email	yuna.choi@einsteinmed.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD072591

PRIDE project URI

• PRIDE
  1. PXD072591
     1. Label: PRIDE project
     2. Name: Proteomics for extracellular vesicles from cultured tanycytes with target knockdown versus control