PXD072579

PXD072579 is an original dataset announced via ProteomeXchange.

Title	Identification of protein interaction partners of SET-25 in Caenorhabditis elegans embryos using FLAG-tagged immunoprecipitation combined with liquid chromatography-tandem mass spectrometry (LC-MSMS)
Description	Histone H3K9 trimethylation (H3K9me3) plays a pivotal role in the establishment and maintenance of heterochromatin and is essential for facilitating epigenetic inheritance. H3K9me3 is often biasedly deposited and associated with repetitive sequence regions, although the underlying mechanism remains poorly understood. In this study, we identify two proteins, SECP-1 and SECP-2, as crucial cofactors for the genome-wide deposition of H3K9me3 in Caenorhabditis elegans. SECP-1 interacts directly with SECP-2 and the primary H3K9me3 methyltransferase, SET-25, forming a protein complex that bridges SECP-2 with SET-25. This complex assembles in the cytoplasm and is transported into the nucleus through SET-25. Once in the nucleus, the complex is recruited to and concentrated at SET-25’s genomic targets in an interdependent manner. The accumulation of this complex correlates with the formation of condensates at repetitive sequences, the perinuclear localization of heterochromatin, and the epigenetic inheritance of small RNA-mediated gene silencing. We propose that repetitive sequences drive the formation of condensates containing H3K9me3 methyltransferase complexes, which in turn causes a biased deposition of H3K9me3 at these genomic regions.
HostingRepository	PRIDE
AnnounceDate	2026-02-25
AnnouncementXML	Submission_2026-02-24_23:27:03.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	haiqian ma
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: NEWT:6239;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-02 07:44:46	ID requested
⏵ 1	2026-02-24 23:27:03	announced

Dataset with its publication pending

submitter keyword: embryo,C.elegans,LC-MSMS

Gang Wan
contact affiliation	Innovation Center for Evolutionary Synthetic Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou, China
contact email	wangang5@mail.sysu.edu.cn
lab head
haiqian ma
contact affiliation	中山大学
contact email	1710812539@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD072579

PRIDE project URI

• PRIDE
  1. PXD072579
     1. Label: PRIDE project
     2. Name: Identification of protein interaction partners of SET-25 in Caenorhabditis elegans embryos using FLAG-tagged immunoprecipitation combined with liquid chromatography-tandem mass spectrometry (LC-MSMS)