PXD072524

PXD072524 is an original dataset announced via ProteomeXchange.

Title	Benchmark dataset relating to Kamrad et al "Interspecies interactions drive bacterial proteome reorganisation and emergent metabolism"
Description	Three co-cultures containing gram-positive and -negative species at different ratios (1:1 to 1:9) were measured in quadruplicates, as well as the same number of corresponding mono-cultures. To isolate LC-MS matrix effects in the absence of biological proteome changes, we mixed mono-culture samples in the same ratio (co-measurement controls). Additionally, we mixed mono-cultures in the same ratio before protein extraction (co-extraction controls) to detect potential artefacts introduced during sample preparation. The results of this experiment are shown in the Extended Data Fig. 1.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-02_09:16:51.257.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Kamrad
SpeciesList	scientific name: Bacteroides thetaiotaomicron; NCBI TaxID: NEWT:818; scientific name: Agathobacter rectalis; NCBI TaxID: NEWT:39491; scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: NEWT:272563;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-30 11:20:54	ID requested
⏵ 1	2026-02-02 09:16:51	announced

Dataset with its publication pending

submitter keyword: gut bacteria, synthetic communities,Microbiome

Kiran R Patil
contact affiliation	MRC Toxicology Unit, University of Cambridge
contact email	kp533@cam.ac.uk
lab head
Stephan Kamrad
contact affiliation	The Francis Crick Institute
contact email	stephan.kamrad@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD072524

PRIDE project URI

• PRIDE
  1. PXD072524
     1. Label: PRIDE project
     2. Name: Benchmark dataset relating to Kamrad et al "Interspecies interactions drive bacterial proteome reorganisation and emergent metabolism"