⮝ Full datasets listing
PXD072480
PXD072480 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Single Cell-Type Spatial Proteomics Uncovers Regional Heterogeneity of Astrocytes |
| Description | Astrocytes are glial cells in the central nervous system (CNS) that support numerous processes essential for brain function. Their functional diversity is thought to arise from specialized subpopulations with distinct molecular profiles. Although single-cell and single-nucleus RNA sequencing (scRNA-seq and snRNA-seq) have greatly advanced our understanding of astrocyte transcriptomic heterogeneity, mRNA abundance does not always correlate with protein levels because of post-transcriptional and translational regulation. To address this limitation, we applied Microscoop Mint, a microscopy-guided spatial proteomics platform that integrates subcellular, region-specific sample preparation with LC-MS/MS-based mass spectrometry, enabling direct protein profiling from formalin-fixed, optimal cutting temperature (OCT)-embedded mouse brain tissue. This approach uncovered distinct astrocyte proteomic signatures in the cerebral cortex and hippocampus and identified region-enriched astrocyte markers, which were subsequently validated by immunofluorescence. Notably, MINK1 and PLEKHB1 showed preferential expression in hippocampal and cortical astrocytes, respectively, highlighting their potential as region-specific astrocyte markers. Overall, this strategy enables high-precision, unbiased spatial proteomic discovery at subcellular resolution, providing a powerful framework for linking molecular diversity to functional specialization in astrocyte biology. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-05-26 |
| AnnouncementXML | Submission_2026-05-26_22:14:16.724.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jung-chi Liao |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Oxidation; Deamidated; Carbamidomethyl; Acetyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-28 22:29:58 | ID requested | |
| ⏵ 1 | 2026-05-26 22:14:17 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Single cell, spatial proteomics, regional heterogeneity, astrocytes, cortex, hippocampus, DatasetType:Proteomics |
Contact List
| Jung-Chi Liao | |
|---|---|
| contact affiliation | Syncell Inc. |
| contact email | liaojc@syncell.com |
| lab head | |
| Jung-chi Liao | |
| contact affiliation | SYNCELL INC |
| contact email | liaojc@syncell.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100345/ |
