PXD072402

PXD072402 is an original dataset announced via ProteomeXchange.

Title	Ptprf is the conserved receptor for Asprosin’s glucogenic effects in vertebrates
Description	Asprosin, a fasting-induced glucogenic hormone, plays a crucial role in maintaining glucose homeostasis; however, its receptor remains elusive. This study identifies protein tyrosine phosphatase receptor F (Ptprf) as the receptor mediating Asprosin's metabolic functions. Using zebrafish models, we demonstrate the evolutionary conservation of Asprosin's role in glucose metabolism. Through binding assays, we determined Ptprf as Asprosin's interacting receptor in zebrafish liver. Zebrafish possess two Ptprf paralogs (Ptprfa/b), both hepatically expressed and binding Asprosin with high affinity. Genetic ablation of ptprfa/b reduced basal glucose levels and eliminated Asprosin-induced hyperglycemia. Conversely, overexpression of soluble Ptprf ligand-binding domains neutralized Asprosin's glucogenic effects. These findings were validated in mammals: Asprosin binds PTPRF in mice and humans, and Ptprf knockout mice showed blunted response to Asprosin. Our results establish Ptprf as an evolutionarily conserved Asprosin receptor across vertebrates, providing mechanistic insights for developing therapies targeting this pathway for diabetes and obesity.
HostingRepository	PRIDE
AnnounceDate	2026-01-12
AnnouncementXML	Submission_2026-01-11_23:02:40.075.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiquan Liu
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: NEWT:7955;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-24 09:14:59	ID requested
⏵ 1	2026-01-11 23:02:40	announced

Dataset with its publication pending

submitter keyword: human, insulin receptor, Ptprf, mouse,Glucose, Asprosin, zebrafish

Jianzhen Li
contact affiliation	College of Life Sciences, Northwest Normal University, Lanzhou 730070, China
contact email	lijianzhen@nwnu.edu.cn
lab head
Zhiquan Liu
contact affiliation	College of Life Sciences, Northwest Normal University
contact email	oldcat1996@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072402
     1. Label: PRIDE project
     2. Name: Ptprf is the conserved receptor for Asprosin’s glucogenic effects in vertebrates