PXD072354

PXD072354 is an original dataset announced via ProteomeXchange.

Title	Development of a 3D in vitro model of Dupuytren’s disease as a platform for drug screening
Description	Background: Dupuytren’s disease (DD) is a common fibrotic disorder of the hand, characterized by progressive thickening and contracture of the palmar and digital fascia. Surgical excision remains the primary treatment; however, there are currently no therapies to prevent disease progression or recurrence. This study aims to develop a 3D in vitro model to test novel antifibrotic therapies. The model is based on decellularized pathological DD tissue seeded with patient-derived fibroblasts, capturing the role of both cellular and extracellular matrix components in disease progression. Methods: Fibrotic DD tissues were obtained from surgical excisions, sectioned, and decellularized. In parallel, primary fibroblasts were isolated from patient samples. The decellularized extracellular matrices (dECMs) were characterized with respect to biochemical composition, collagen structure, and mechanical properties. Fibroblasts were seeded onto the dECMs and cultured stepwise to initially promote proliferation, followed by differentiation into myofibroblasts. Secretomes of cells cultivated on the established 3D model were compared to those from conventional 2D cultivations. To evaluate the model´s relevance and effectiveness we tested the antifibrotic drug minoxidil. Results: The dECMs retained the pathological architecture and mechanical properties of native DD tissue, although individual ECM components were reduced after decellularization. Fibroblasts successfully adhered, proliferated, and repopulated the scaffold. The relevance of the 3D model was demonstrated by the presence of myofibroblasts with disease–relevant secretome. The responsiveness to the drug minoxidil was significantly more complex in the 3D model than in conventional 2D cultures. Conclusion: We demonstrated that dECM seeded with DD fibroblasts represents a relevant 3D in vitro model of Dupuytren’s disease. The model enables antifibrotic drug screening, as demonstrated by the testing of minoxidil. Our model provides a reproducible platform also suitable for the investigation of cells and ECM contributions to palmar fascial fibrosis.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_16:36:13.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adam Eckhardt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-23 09:02:40	ID requested
⏵ 1	2026-04-05 16:36:14	announced

10.1007/s12195-026-00885-2;

Knitlova J, Eckhardt A, Hadraba D, Vondrasek D, Stachon R, Filova E, Jencova V, Havlickova K, Kobets T, Ostadal M, Bacakova L, Development of a 3D In Vitro Model of Dupuytren's Disease as a Platform for Drug Screening. Cell Mol Bioeng, 19(1):111-127(2026) [pubmed]

ProteomeXchange project tag: Skeletal Muscle Proteome (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: myofibroblasts, proteomics,Dupuytren’s disease, 3D in vitro model, fibrosis, minoxidil, collagen type I, decellularization

Adam Eckhardt
contact affiliation	Institute of Physiology, Czech Academy of Sciences
contact email	adam.eckhardt@fgu.cas.cz
lab head
Adam Eckhardt
contact affiliation	Institute of Physiology AVCR
contact email	adam.eckhardt@fgu.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD072354

PRIDE project URI

• PRIDE
  1. PXD072354
     1. Label: PRIDE project
     2. Name: Development of a 3D in vitro model of Dupuytren’s disease as a platform for drug screening