PXD072299

PXD072299 is an original dataset announced via ProteomeXchange.

Title	Fallopian tube lavage sampling for early detection of pre-invasive ovarian cancer
Description	Background Ovarian cancer (OC) is the most lethal gynaecological malignancy, primarily due to late-stage diagnosis. Most high-grade serous OC cases arise from pre-invasive fallopian tube (FT) lesions, known as serous tubal intraepithelial carcinomas (STICs), yet no clinical test exists for detecting these at an early, treatable stage. Here, we introduce FT lavage as a novel sampling approach that avoids the fimbrial ends, enabling analysis of early tumour-associated molecular changes in FTderived biofluids, while remaining compatible with emerging in vivo sampling tools. Methods Proteomic profiling was performed on FT lavage samples from high-risk BRCA1/2 mutation carriers, OC patients, and control individuals with benign gynaecological conditions. A subset of the identified proteins was assessed by immunofluorescent staining in STIC lesions from an independent cohort. Results We identified an 82-protein signature featuring key factors implicated in OC progression and dysregulated in early disease. Using this signature, we retrospectively identified a previously undetected STIC lesion in a high-risk patient. Immunofluorescent staining confirmed dysregulation of select proteins in STIC lesions. Notably, detection of this signature away from the fimbrial region suggests molecular changes occur throughout the FT lumen, including proximal areas, making this approach accessible via next-generation falloposcopy platforms. Conclusions Our findings support FT lavage sampling as a minimally invasive method for detecting premalignant lesions in high-risk individuals, with potential for fertility-preserving early detection. This approach could inform screening, risk stratification, and chemoprevention strategies. Larger studies are needed to validate its diagnostic performance and clinical utility for asymptomatic screening.
HostingRepository	PRIDE
AnnounceDate	2026-01-07
AnnouncementXML	Submission_2026-01-07_04:35:01.474.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD072299
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sarah Harbach
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-22 05:55:00	ID requested
⏵ 1	2026-01-07 04:35:01	announced

10.6019/PXD072299;

10.1002/CTM2.70557;

submitter keyword: Human, fallopian tube lavage, High-Grade Serous Ovarian Cancer (HGSOC), LC-MS/MS

Christine Schmidt
contact affiliation	Genomic Stability Lab (GSL), Manchester Cancer Research Centre, Division of Cancer Sciences, Faculty of Biology Medicine and Health, University of Manchester, UK
contact email	christine.schmidt@manchester.ac.uk
lab head
Sarah Harbach
contact affiliation	University of Manchester
contact email	sarah.harbach@manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072299
     1. Label: PRIDE project
     2. Name: Fallopian tube lavage sampling for early detection of pre-invasive ovarian cancer