PXD072296

PXD072296 is an original dataset announced via ProteomeXchange.

Title	Identification of novel blood-borne soluble binding partners of factor H-related proteins
Description	The deposition of circulating complement factor H-related (FHR) proteins in tissues around the body has been implicated in a series of complement-mediated diseases. However, the array of blood-borne binding partners with which they interact remains unclear. Here, we identify novel blood-borne binding partners of FHR proteins using untargeted immunoprecipitation and mass spectrometry. We uncover direct interactions between FHRs and soluble immune mediators including complement C4, cathepsin G (CTSG), mannose-binding lectin 2 (MBL2), platelet basic protein (PPBP), and fibrinogen-like protein 1 (FGL1). Functional assays show that FHR-1 and FHR-2 attenuate CTSG-mediated C3b degradation, while FHR-5 and FHL-1 appear to affect lectin pathway activation via MBL2 binding. These interactions suggest that FHRs perhaps confer activity not only through surface competition with factor H, but also via selective engagement with circulating ligands. Our findings expand the known FHR interactome and provide mechanistic insight into their accumulation at inflammatory sites within the ECM (extracellular matrix). This work reveals potential new avenues for understanding FHR biology and targeting complement dysregulation in disease.
HostingRepository	PRIDE
AnnounceDate	2026-03-30
AnnouncementXML	Submission_2026-03-29_17:46:06.450.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tina Beyer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-22 05:18:04	ID requested
⏵ 1	2026-03-29 17:46:07	announced

10.1038/s41598-026-44779-9;

Tang J, Woerz F, Beyer T, Boldt K, Rathi S, Zhao S, Wolfram L, Ueffing M, Clark SJ, Identification of novel blood-borne soluble binding partners of factor H-related proteins. Sci Rep, 16(1):(2026) [pubmed]

submitter keyword: FHR, affinity proteomics, blood

Prof. Dr. Simon Clark
contact affiliation	Institute for Ophthalmic Research, University of Tuebingen
contact email	simon.clark@uni-tuebingen.de
lab head
Tina Beyer
contact affiliation	Uniklinik Tübingen
contact email	tina.beyer@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD072296

PRIDE project URI

• PRIDE
  1. PXD072296
     1. Label: PRIDE project
     2. Name: Identification of novel blood-borne soluble binding partners of factor H-related proteins