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PXD072282
PXD072282 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ligilactobacillus murinus alleviates atherosclerosis in mice by promoting 7DHCA mediated intestinal stem cells proliferation and gut barrier repair |
| Description | Multiple studies suggest that the gut microbiota may play a crucial role in the development and progression of atherosclerosis (AS). The present study was initiated on the anti-atherosclerotic effect of the Gualou–Xiebai (GLXB) herb pair. However, the exact mechanisms underlying the beneficial effects of GLXB on AS remain poorly understood. ApoE-/- mice were fed a high-fat diet (HFD) to establish an AS model. Fecal microbiota transplantation (FMT) experiments confirmed the microbiota-dependent effects of GLXB. Metagenomic sequencing and metabolomics were used to screen for the key strains and the differential metabolites. Key strains were then administered to AS mice via gavage. Finally, the proliferation of intestinal stem cells (ISCs) was observed using small intestinal organoids in vitro. Our findings identified Ligilactobacillus murinus (L. murinus) as a key GLXB-regulated strain and a major bile acid producer. Supplementation with live L. murinus (live L. m) significantly alleviated AS progression, improved dyslipidemia, reduced inflammation, and mitigated gut barrier damage in HFD-fed mice. Metabolomics identified bile acids as key differential metabolites following L. murinus supplementation. Furthermore, molecular docking revealed that multiple L. murinus-regulated bile acids can bind to the bile acid receptor G protein-coupled bile acid receptor 1 (GPBAR1, also called TGR5), with 7-dehydrocholic acid (7-DHCA) showing the strongest affinity. Consistently, L. murinus supplementation elevated serum 7-DHCA levels and up-regulated TGR5 mRNA expression. Moreover, in small intestinal organoids, 7-DHCA supplementation significantly enhanced the mRNA expression of SRC, YAP, and their downstream target Ctgf, while increasing Lgr5 and occludin expression. These findings suggested that GLXB-enriched L. murinus alleviated AS in mice, potentially via 7-DHCA production and subsequent gut barrier repair. |
| HostingRepository | iProX |
| AnnounceDate | 2025-12-21 |
| AnnouncementXML | Submission_2025-12-22_00:37:12.044.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Luyao Zhao |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-22 00:36:54 | ID requested | |
| ⏵ 1 | 2025-12-22 00:37:12 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Gut microbiota, Atherosclerosis, Ligilactobacillus murinus, Bile acids, Gut barrier, Gualou-Xiebai herb pair |
Contact List
| Yarong Liu | |
|---|---|
| contact affiliation | Anhui University of Chinese Medicine |
| contact email | lyr891209@163.com |
| lab head | |
| Luyao Zhao | |
| contact affiliation | Anhui University of Chinese Medicine |
| contact email | 1215161923@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
