PXD072271

PXD072271 is an original dataset announced via ProteomeXchange.

Title	Mapping of the local proteome of the Arabidopsis chloroplast intermembrane space by proximity labelling
Description	The chloroplast intermembrane space, located between the outer and inner envelope membranes, plays key roles in protein and lipid trafficking. Currently, the proteome of the intermembrane space is largely uncharacterized, with only a relatively few protein players identified, and thus limiting our understanding of the functional relevance of this region. Here, we used a biotin proximity labelling technique, based on the TurboID enzyme (a modified biotin ligase), to define the chloroplast intermembrane space proteome in Arabidopsis thaliana. TurboID was targeted to the intermembrane space when fused with the chloroplast transit peptide (cTP) of A. thaliana Tic22, a 22-kDa protein component of the translocon at the inner chloroplast membrane. Localization of the Tic22cTP fusion protein in the intermembrane space was confirmed by confocal microscopy and protease protection assays of isolated intact chloroplasts. Following optimization of biotinylation, mass spectrometry analysis revealed a distinct set of candidate intermembrane space-localized proteins, including known components of protein import and lipid trafficking pathways, as well as several other novel proteins.
HostingRepository	PRIDE
AnnounceDate	2026-07-13
AnnouncementXML	Submission_2026-07-13_14:16:23.782.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-21 20:53:09	ID requested
1	2026-07-08 11:39:45	announced
⏵ 2	2026-07-13 14:16:24	announced	2026-07-13: Updated project metadata.

10.1093/jxb/erag348;

submitter keyword: IMS, LC-MSMS,chloroplast

Yang Xu
contact affiliation	Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada
contact email	yangxu@uoguelph.ca
lab head
Yang Xu
contact affiliation	University of Guelph
contact email	yangxu@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072271
     1. Label: PRIDE project
     2. Name: Mapping of the local proteome of the Arabidopsis chloroplast intermembrane space by proximity labelling