PXD072246

PXD072246 is an original dataset announced via ProteomeXchange.

Title	Mass Spectrometry-Based Peptidomics for Discovery and Profiling of Antimicrobial and Neuropeptide in Crustacean Hemolymph
Description	Crustacean hemolymph contains a diverse profile of bioactive peptides, including antimicrobial peptides (AMPs) and neuropeptides, which regulate essential immune and physiological processes. However, rapid coagulation and high-abundance proteins like hemocyanin complicate mass spectrometry-based peptidomics analysis. We systematically evaluated five extraction workflows to optimize endogenous peptide recovery from the hemolymph of the American lobster (Homarus americanus) and the blue crab (Callinectes sapidus). Among the tested approaches, Method 3, incorporating an anticoagulant buffer with protease inhibitors followed by 10 kDa molecular weight cutoff filtration, provided the highest peptide ID recovery. This workflow minimized clotting-related peptide loss and enzymatic degradation, yielding significantly greater numbers of AMPs in both species. Notably, omitting the reduction and alkylation protocol in Method 3 was superior for AMPs identification. For example, in C. sapidus, adding these steps (Method 4) resulted in a 40% decrease in AMPs IDs, likely due to increased sample handling and incubation times. Method 3 also maximized neuropeptide recovery in H. americanus, identifying mature orcokinins and myosuppressins. In C. sapidus, neuropeptide recovery varied across methods with Method 3 showing competitive performance. Additionally, we reported six novel putative C. sapidus neuropeptides, and identified 11 and 12 in silico predicted novel AMPs in C. sapidus and H. americanus, respectively, characterized by amino acid motifs. Collectively, this study demonstrated an integrated extraction strategy to enable more comprehensive profiling of crustacean hemolymph peptidomes and provided the first report of AMPs, alongside neuropeptides, in C. sapidus and H. americanus, expanding the repertoire of candidates for antimicrobial therapeutics and neuromodulation.
HostingRepository	MassIVE
AnnounceDate	2026-05-19
AnnouncementXML	Submission_2026-05-19_14:14:28.477.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huong Tran
SpeciesList	scientific name: Homarus americanus; common name: American lobster; NCBI TaxID: 6706; scientific name: Callinectes sapidus; common name: blue crab; NCBI TaxID: 6763;
ModificationList	Amidated; Oxidation; Glu->pyro-Glu; Gln->pyro-Glu
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-20 13:58:33	ID requested
⏵ 1	2026-05-19 14:14:28	announced

no publication

submitter keyword: antimicrobial peptides, neuropeptides, peptidomics, crustacean, hemolymph, mass spectrometry, DatasetType:Proteomics

Lingjun Li
contact affiliation	University of Wisconsin-Madison
contact email	lingjun.li@wisc.edu
lab head
Huong Tran
contact affiliation	University of Wisconsin - Madison
contact email	ngochuongaug01@gmail.com
dataset submitter

MassIVE dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100293/