PXD072239

PXD072239 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome modulation by zinc and iron in Klebsiella pneumoniae reveals new virulence mechanisms and antibacterial targets
Description	Klebsiella pneumoniae is a Gram-negative bacterium causing 10-20% mortality in immunocompromised individuals and is known to rapidly develop resistance towards existing antibiotics. This underscores a need for novel antimicrobials or strategies for resensitization. Bacterial adaptation to iron and zinc, which are key nutrients linked to growth, division, and virulence, offers promising targets for antibiotic intervention. In this thesis, mass spectrometry-based phosphoproteomics was used to profile K. pneumoniae in iron/zinc-replete and -limited media, identifying 574 phosphopeptides from 358 phosphoproteins. Two candidates, SbmA and TolQ, were selected for disruption based on hypothesized roles in iron acquisition and membrane stabilization, respectively. Through iron quantification assays, growth curves, electron microscopy, antibiotic susceptibility testing and infection assays, we identified a previously uncharacterized role for SbmA in iron acquisition and identified reduced growth, viability, increased β-lactam susceptibility, and enhanced immune clearance for ΔtolQ. High-throughput drug screening revealed two compounds that target SbmA or TolQ, reducing bacterial growth and enhance immune clearance. Together, these findings highlight phosphoproteomics as a powerful tool for identifying nutrient-regulated targets and position TolQ and SbmA as promising candidates for antimicrobial development.
HostingRepository	PRIDE
AnnounceDate	2026-04-15
AnnouncementXML	Submission_2026-04-14_17:29:52.752.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chelsea Reitzel
SpeciesList	scientific name: Klebsiella pneumoniae subsp. pneumoniae; NCBI TaxID: NEWT:72407;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-19 22:22:33	ID requested
⏵ 1	2026-04-14 17:29:53	announced

Dataset with its publication pending

submitter keyword: pneumoniae, Exploris, phosphorylation, bacteria, phosphoproteomics, iron,klebsiella, zinc

Jennifer Geddes-McAlister
contact affiliation	Molecular and Cellular Biology, University of Guelph, Canada
contact email	jgeddesm@uoguelph.ca
lab head
Chelsea Reitzel
contact affiliation	University of Guelph
contact email	reitzelc@uoguelph.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD072239

PRIDE project URI

• PRIDE
  1. PXD072239
     1. Label: PRIDE project
     2. Name: Phosphoproteome modulation by zinc and iron in Klebsiella pneumoniae reveals new virulence mechanisms and antibacterial targets