PXD072220 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Copper-responsive proteome changes in human kidney proximal tubule epithelial cells |
| Description | Copper is essential for cellular function but potentially toxic in excess. While copper's influence on cytoskeletal dynamics remains unclear, loss of mechanical integrity is a hallmark of kidney disease. Here we investigated how copper availability modulates mechanical properties and cytoskeletal architecture of human proximal tubule epithelial cells (HK-2). Cells were treated for 24h with 50 µM CuCl₂ (copper supplementation) or 10 µM tetrathiomolybdate (TTM, copper chelation). Quantitative proteomics revealed coordinated changes in actin-regulatory proteins including Rab35, Septin2, MsrB2, and CK2 subunits, establishing redox-dependent pathways linking copper homeostasis to cytoskeletal dynamics. A total of 4,933 proteins were quantified with 89-90% data completeness, including detection of 13,919 PTMs per run (carbamidomethylation, oxidation, phosphorylation sites) |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-21 |
| AnnouncementXML | Submission_2026-07-21_13:06:54.827.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rafael Valverde |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-19 15:09:16 | ID requested | |
| ⏵ 1 | 2026-07-21 13:06:55 | announced | |
Publication List
Keyword List
| submitter keyword: proteomics, cytoskeleton, mechanotransduction, actin, kidney,copper, oxidative stress, HK-2 |
Contact List
| Marcelo Einicker Lamas |
|---|
| contact affiliation | Laboratório de Biomembranas, Instituto de Biofísica Carlos Chagas Filho - Universidade Federal do Rio de Janeiro, RJ, Brazil |
| contact email | einicker@biof.ufrj.br |
| lab head | |
| Rafael Valverde |
|---|
| contact affiliation | Instituto de Biofísica Carlos Chagas Filho - Universidade Federal do Rio de Janeiro |
| contact email | valverde@biof.ufrj.br |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD072220
- Label: PRIDE project
- Name: Copper-responsive proteome changes in human kidney proximal tubule epithelial cells
