⮝ Full datasets listing
PXD072196
PXD072196 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Directed evolution of a genetically encoded photocatalyst for temporally resolved proximity labeling of subcellular RNAs and proteins |
| Description | In eukaryotic cells, the precise spatial localization of RNAs and proteins is essential for proper cellular function. Genetically encoded photocatalytic proximity labeling techniques have expanded our ability to map subcellular proteomes and transcriptomes, but their temporal resolution remains limited. Here, we introduce Lantern, an engineered flavoprotein optimized via directed evolution, which enables sub‑minute, spatially resolved labeling of cellular biomolecules. Lantern is targetable to diverse subcellular compartments, including the endoplasmic reticulum, mitochondria, and stress granules (SGs), to map local transcriptomes (CAP-seq) and proteomes (CAP-MS). Using Lantern, we observed that m6A‑enriched RNAs are recruited to SGs within five minutes of stress induction, while ER‑proximal RNAs associate with the SG scaffold protein G3BP1 during early SG assembly. Additionally, Lantern was adapted for cell-surface tagging (CAP-CELL), enabling spatially resolved cell typing and the analysis of cell-cell interactions. Collectively, this study establishes Lantern as a powerful tool that offers unprecedented temporal resolution for investigating the dynamic organization of subcellular molecular networks. |
| HostingRepository | iProX |
| AnnounceDate | 2025-12-18 |
| AnnouncementXML | Submission_2026-08-23_19:37:24.902.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuxin Fang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-19 01:44:30 | ID requested | |
| ⏵ 1 | 2026-08-23 19:37:25 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Stress granule, Photocatalytic proximity labeling, Subcellular proteome |
Contact List
| Peng Zou | |
|---|---|
| contact affiliation | Peking University |
| contact email | zoupeng@pku.edu.cn |
| lab head | |
| Yuxin Fang | |
| contact affiliation | Peking University |
| contact email | fangyx201@pku.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
