PXD072143 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Extracellular vesicles isolated from frozen whole blood show biomarker potential in small intestine neuroendocrine neoplasms |
| Description | Despite widespread storage of frozen whole blood (FWB) in biobanks worldwide, including collections from low-income countries and rare cancers, its suitability for extracellular vesicle (EV) research remains largely unassessed. We aimed to investigate the feasability of EV isolation from this resource. In this work, we develop a robust, practical workflow for cancer biomarker analysis of EVs isolated from FWB, by systematically comparing differential ultracentrifugation (dUC), size-exclusion chromatography (SEC), and their combinations. All methods employed resulted in vesicles of size, morphology and marker expression profile consistent with small EV. We then extend our workflow to isolate and profile EVs from FWB of small intestinal neuroendocrine neoplasms (siNEN), to elucidate their potential for biomarker discovery. Proteomics by mass-spectrometry showed elevation of markers and pathways of associated with the neuroendocrine origin of the EVs, highlighting the potential in retrospective biomarker studies of FWB-derived EVs
In a separate experiment we investigated the FWB EVs isolated by dUC, with the EV-depleted supernatant fraction from dUC. Supernatant fractions showcased enrichment of soluble plasma-accodiated proteins and immune-related markers whereas FWB EVs showcased relative enrichment of cell-associated and signaling related proteins |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-19 |
| AnnouncementXML | Submission_2026-06-19_02:13:21.467.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jonas Burman |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; Carbamidomethyl; oxidized residue |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-18 06:55:08 | ID requested | |
| ⏵ 1 | 2026-06-19 02:13:21 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
Contact List
| Linda Bojmar |
|---|
| contact affiliation | Department of Biomedical and Clinical Sciences, Bojmar Lab, Linköping University, Linköping, Sweden |
| contact email | linda.bojmar@liu.se |
| lab head | |
| Jonas Burman |
|---|
| contact affiliation | Linköping University |
| contact email | jonas.burman@liu.se |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD072143 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD072143
- Label: PRIDE project
- Name: Extracellular vesicles isolated from frozen whole blood show biomarker potential in small intestine neuroendocrine neoplasms
