PXD072142

PXD072142 is an original dataset announced via ProteomeXchange.

Title	REV7 associates with ATRIP and FAM35A
Description	REV7 is a remarkably multi-faceted protein with important and separable roles in multiple genome maintenance pathways including Trans-Lesion Synthesis, the Fanconi Anemia pathway, as well as p53 signaling and Double Stranded Break resection. To investigate novel functions and pathways involving REV7, we performed mass-spectrometry analysis and identified proteins associated with REV7. We discovered two novel factors: the ATR-ATRIP complex and FAM35A. The REV7-FAM35A interaction was reported in a previous paper. Here, we identified the ATR-binding partner and activator ATRIP as a new REV7-associated protein. Through a comprehensive series of in vitro biochemical studies and cell-based assays we demonstrate that ATRIP is a direct binding partner of REV7, and that REV7-ATRIP interactions are critical for negative regulation of ATR activity. Our findings establish a new mechanism for negative regulation of ATR by the DNA repair protein REV7.
HostingRepository	PRIDE
AnnounceDate	2026-01-22
AnnouncementXML	Submission_2026-01-22_09:52:20.026.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junya Tomida
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Velos Pro; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-18 06:49:35	ID requested
⏵ 1	2026-01-22 09:52:21	announced

10.1093/nar/gkaf1527;

Biller M, Kabir S, Nipper S, Allen S, Kayali Y, Kuncik S, Sasanuma H, Zhou P, Vaziri C, Tomida J, REV7 associates with ATRIP and inhibits ATR kinase activity. Nucleic Acids Res, 54(2):(2026) [pubmed]

Biller M, Kabir S, Nipper S, Allen S, Kayali Y, Kuncik S, Sasanuma H, Zhou P, Vaziri C, Tomida J, REV7 associates with ATRIP and inhibits ATR kinase activity. bioRxiv, ():(2025) [pubmed]

10.1101/2025.01.17.633588;

Tomida J, Takata KI, Bhetawal S, Person MD, Chao HP, Tang DG, Wood RD, damage responses of normal and BRCA1-defective cells. EMBO J, 37(12):(2018) [pubmed]

10.15252/embj.201899543;

submitter keyword: ATRIP,REV7, FAM35A

Junya Tomida
contact affiliation	Department of Biological Sciences, Tomida Lab, University of North Carolina at Charlotte, USA
contact email	jtomida@charlotte.edu
lab head
Junya Tomida
contact affiliation	University of North Carolina at Charlotte
contact email	jtomida@charlotte.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD072142

PRIDE project URI

• PRIDE
  1. PXD072142
     1. Label: PRIDE project
     2. Name: REV7 associates with ATRIP and FAM35A