PXD072100

PXD072100 is an original dataset announced via ProteomeXchange.

Title	Mass Spectrometry Proteomics of Engineered Yarrowia lipolytica Strains for Enhanced Carotenoid and Lipid Production Across Two Growth Phases.
Description	This submission contains the mass spectrometry proteomics dataset collected from the oleaginous yeast Yarrowia lipolytica. The data was collected from three strains grown in glycerol mineral medium: a parental control strain (PAR), a genetically modified carotenoid-producing strain (CAR), and a genetically modified lipid-producing strain (OBE). Samples were taken during two distinct physiological states: the initial exponential growth phase and the subsequent nitrogen-limited (Nlim) phase. The proteomics data was primarily used to constrain a newly reconstructed enzyme-constrained genome-scale model (ecGEM) of Y. lipolytica. The analysis provides insights into the physiological states of the engineered strains across the different phases and helped to assess the impact of genetic modifications on the production of target molecules. An average of 4,329 unique proteins were detected. The mass spectrometry acquisition utilized a Data Independent Analysis (DIA) method , and the raw data was processed using Dia-NN software.
HostingRepository	PRIDE
AnnounceDate	2026-03-30
AnnouncementXML	Submission_2026-03-30_05:01:46.649.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliano Sabedotti De Biaggi
SpeciesList	scientific name: Yarrowia lipolytica (Candida lipolytica); NCBI TaxID: NEWT:4952;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-17 08:36:10	ID requested
⏵ 1	2026-03-30 05:01:47	announced

De Biaggi JS, Park YK, Kerkhoven EJ, Ledesma-Amaro R, Lahtvee PJ, Enzyme-constrained genome-scale model of Yarrowia lipolytica predicts growth-phase specific metabolic engineering targets. Appl Microbiol Biotechnol, 110(1):(2026) [pubmed]

10.1007/s00253-026-13791-4;

submitter keyword: Glycerol

Petri-Jaan Lahtvee
contact affiliation	Department of Chemistry and Biotechnology, Talltech, Tallinn, Estonia
contact email	lahtvee@taltech.ee
lab head
Juliano Sabedotti De Biaggi
contact affiliation	Taltech
contact email	juliano.sabedotti@taltech.ee
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072100
     1. Label: PRIDE project
     2. Name: Mass Spectrometry Proteomics of Engineered Yarrowia lipolytica Strains for Enhanced Carotenoid and Lipid Production Across Two Growth Phases.