PXD072086

PXD072086 is an original dataset announced via ProteomeXchange.

Title	The proteomics of exosomes derived from MSCs
Description	Proteomic profiling of MSC-derived EVs was performed on donor-matched samples. EV proteins were extracted in RIPA buffer containing a complete protease inhibitor cocktail, and total protein concentrations were quantified by BCA assay. Equal protein inputs (20–30 µg) were reduced, alkylated, and subjected to overnight trypsin digestion to generate MS-compatible peptide mixtures following established EV-proteomics workflows. Peptides were resolved by nano-LC and analyzed on a high-resolution Orbitrap mass spectrometer operated in data-dependent acquisition mode. Raw spectra were processed using standard pipelines, with peptide–spectrum matching against the human UniProt reference proteome using a target–decoy strategy. Protein identifications were restricted to a 1% false discovery rate at the peptide and protein levels and required ≥1 unique peptide, consistent with current mass-spectrometry reporting standards.
HostingRepository	iProX
AnnounceDate	2025-12-17
AnnouncementXML	Submission_2026-06-22_00:32:17.302.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuiqin Fan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-17 01:33:35	ID requested
⏵ 1	2026-06-22 00:32:17	announced

Dataset with its publication pending

submitter keyword: MSCs, exosome, proteomics

Lian Ma
contact affiliation	West China Second Hospital of Sichuan University
contact email	malian8965@163.com
lab head
Chuiqin Fan
contact affiliation	West China Second Hospital of Sichuan University
contact email	19cqfan@alumni.stu.edu.cn
dataset submitter

iProX dataset URI