PXD072074

PXD072074 is an original dataset announced via ProteomeXchange.

Title	Food Dependent Activation of One Carbon Metabolism During Heat Shock in Mytilus californianus
Description	Of the ecological factors affecting the cellular stress response (CSR), food ration has received little attention despite a likely role in stress tolerance limits. To test the interactive effects of food and temperature on the proteomic response to heat shock, the intertidal mussel Mytilus californianus was acclimated to four combinations of nearshore (low) and aquaculture (high) phytoplankton levels, combined with low (20 °C) and high (30 °C) aerial temperatures during daytime low tides. Whole mussels were then exposed to an acute (6 h), aerial heat stress (33 °C) and allowed to recover for 1 h and 24 h in pre-exposure conditions. Proteomic changes in the gill before and after heat shock were measured using liquid-chromatography-mass spectrometry and label-free quantification. Mussels acclimated to low-temperature-low-food (LTLF) elicited a greater heat shock response compared to other acclimation groups, increasing protein levels of core carbohydrate and sulfide metabolism. These glycolytic metabolites proposedly feed into one-carbon metabolism (e.g., folate and methionine cycles) and connecting pathways, which also increased abundance and likely contribute to purine synthesis, methylation, and transsulfuration. These mussels additionally increased levels of proteins scavenging hydrogen peroxide, chelating ferrous iron, and maintaining reduced glutathione levels. Proteins modifying chromatin (histone) structure and regulating transcription likely link the metabolic changes to chromatin modifications, specifically through SAM-dependent methyltransferases, which may contribute to repairing DNA-damage caused by oxidative stress. Overall, our results suggest a food-dependent link between metabolism and epigenetic modifications that we hypothesize may function as an autoregulatory feedback mechanism during the CSR under tidally fluctuating conditions. SUMMARY STATEMENT A food-dependent link between metabolism and epigenetic modifications may function as an autoregulatory feedback mechanism to repair DNA damage and scavenge oxidants during heat stress.
HostingRepository	PRIDE
AnnounceDate	2026-07-28
AnnouncementXML	Submission_2026-07-28_03:25:58.212.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD072074
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Frank Fabela
SpeciesList	scientific name: Mytilus californianus; NCBI TaxID: NEWT:6549;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-16 19:41:12	ID requested
⏵ 1	2026-07-28 03:25:58	announced

10.6019/PXD072074;

10.1242/JEB.252105;

submitter keyword: cellular stress response, proteomics,carbohydrate metabolism, Mytilus, oxidative stress, one carbon metabolism, histone modifications

Lars Tomanek
contact affiliation	California Polytechnic State University San Luis Obispo ;Biological Sciences Department ; California Unites States
contact email	ltomanek@calpoly.edu
lab head
Frank Fabela
contact affiliation	California Polytechnic State University San Luis Obispo
contact email	fabela.frank@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072074
     1. Label: PRIDE project
     2. Name: Food Dependent Activation of One Carbon Metabolism During Heat Shock in Mytilus californianus