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PXD072068
PXD072068 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Nuclear Proteomics in Senescent Cells via NCP-Mediated Photocatalytic Proximity Labeling |
| Description | Spatiotemporal profiling of nuclear-associated proteomes is crucial for elucidating disease mechanisms, identifying key therapeutic targets1-2, and guiding the design of effective drugs. Currently, proximity labeling (PL) using genetically transfected enzymes or photocatalyst-based probes has emerged as a powerful tool for proteomic mapping. However, these approaches are limited by their incompatibility with hard-to-transfect cells and primary tissues, as well as by the lack of efficient nucleus-targeting strategies. In this study, we developed a photocatalytic proximity labeling strategy (Pc-PL) that enables efficient enrichment of nuclear-associated proteins by combining a nucleus-targeted photosensitizer (NCP) with photocatalysis-mediated reactive biotin labeling. Compared with traditional photocatalysts such as chlorin e6 and rose bengal, NCP exhibited superior nuclear accumulation across various cell types. Cellular experiments confirmed that NCP-mediated photoactivation precisely localized biotin labeling within the nucleus, enabling selective enrichment of nuclear proteins via subsequent streptavidin-based magnetic capture. Coupling Pc-PL with quantitative mass spectrometry enabled high-resolution mapping of nuclear proteomes and led to the discovery of previously unrecognized senescence-associated regulators, including TMPO. Collectively, these findings establish Pc-PL as an innovative and versatile tool for high-resolution nuclear proteomics, offering broad potential for target discovery and drug development. |
| HostingRepository | iProX |
| AnnounceDate | 2025-12-16 |
| AnnouncementXML | Submission_2026-03-01_17:56:54.823.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hang Shi |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-16 19:00:09 | ID requested | |
| ⏵ 1 | 2026-03-01 17:56:55 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: senescent cell, Nuclear Proteomics |
Contact List
| Duo Mao | |
|---|---|
| contact affiliation | Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-Sen University |
| contact email | maod6@mail.sysu.edu.cn |
| lab head | |
| Hang Shi | |
| contact affiliation | Institute of Precision Medicine, The First Affiliated Hospital of Sun Yat-Sen University |
| contact email | shpk318u@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
