PXD072063

PXD072063 is an original dataset announced via ProteomeXchange.

Title	Association of tear fluid glutathione synthetase and glutathione levels with amyloid positivity
Description	Tear fluid is a promising source of biomarkers for Alzheimer’s disease (AD). This study characterized the tear fluid proteome across AD and dementia stages. Tear fluid was collected from 60 participants, cognitively normal (CN) individuals (n=32), patients with mild cognitive impairment (n=14), and dementia (n=14). Of these, 27 were amyloid PET-negative and 33 PET-positive. The proteome was analyzed using label-free mass spectrometry, followed by targeted validation immunoassays of key findings. In total, 703 proteins were reliably identified. A notable upregulated protein in dementia compared with CN was glutathione synthetase (GSS) (1.7-fold, p = 0.02). Validation revealed elevated GSS (2-fold) and its product glutathione (1.7-fold) in tear fluid of amyloid PET–positive participants. Incorporating GSS and glutathione as pre-screeners could reduce the need for PET scans by up to 75%. Tear fluid shows promise as a non-invasive source for AD biomarkers, offering novel disease insights, and potential utility for pre-screening prior to PET imaging.
HostingRepository	PRIDE
AnnounceDate	2026-03-25
AnnouncementXML	Submission_2026-03-25_15:48:46.898.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ashok Sharma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-16 16:25:43	ID requested
⏵ 1	2026-03-25 15:48:47	announced

Dataset with its publication pending

submitter keyword: Tear fluid, amyloid PET, Alzheimer’s disease, Dementia, Glutathione synthetase, Diagnosis, Proteomics, Immunoassay, Biomarkers

Ashok Sharma
contact affiliation	Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Augusta University, Augusta, GA, UnitedStates
contact email	assharma@augusta.edu
lab head
Ashok Sharma
contact affiliation	Augusta University
contact email	assharma@augusta.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072063
     1. Label: PRIDE project
     2. Name: Association of tear fluid glutathione synthetase and glutathione levels with amyloid positivity