PXD071955

PXD071955 is an original dataset announced via ProteomeXchange.

Title	A Chemoproteomic Atlas of the Human Purine Interactome for Regioselective Ligand Discovery
Description	Purines are essential bioactive molecules that interact with a large fraction of the human proteome. Despite their importance, the scope of actionable purine-binding pockets for ligand discovery remains limited. Here, we developed a quantitative chemoproteomics platform using sulfonyl-purine (SuPUR) chemistry to produce a massive and functional map of the human purine interactome. The SuPUR platform captured 31,000+ targetable tyrosine and lysine sites, representing the most comprehensive beyond cysteine chemoproteomics database for enabling protein ligand discovery. SuPUR ligands that bind through a regioselective fashion serve as enabling starting points for developing potent (nanomolar) and proteome-wide-selective modulators of enzymatic and protein-protein interaction function. Phenotypic screening identified a site-specific (Y237) and regioselective SuPUR ligand of ACAT2 to reveal an unexpected metabolic dependency in cancer cells. A crystal structure of SuPUR ligand-bound ACAT2 revealed the purine group binds deep in the CoA pocket forming key interactions with catalytic residues via a water bridge to guide future structure-based ligand design
HostingRepository	PRIDE
AnnounceDate	2026-03-29
AnnouncementXML	Submission_2026-03-28_20:43:09.671.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD071955
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ku-Lung Hsu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-13 11:00:28	ID requested
⏵ 1	2026-03-28 20:43:10	announced

10.6019/PXD071955;

submitter keyword: None

Ku-Lung Hsu
contact affiliation	1Department of Chemistry, University of Texas at Austin, Austin, TX, 78712, USA
contact email	ken.hsu@austin.utexas.edu
lab head
Ku-Lung Hsu
contact affiliation	The University of Texas at Austin
contact email	ken.hsu@austin.utexas.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD071955

PRIDE project URI

• PRIDE
  1. PXD071955
     1. Label: PRIDE project
     2. Name: A Chemoproteomic Atlas of the Human Purine Interactome for Regioselective Ligand Discovery