PXD071931

PXD071931 is an original dataset announced via ProteomeXchange.

Title	Kinome profiling of BV2 cells treated with crizotinib or compound 32
Description	Src homology 2 domain–containing inositol 5-phosphatase 1 (SHIP1), encoded by gene INPP5D, is a lipid phosphatase that negatively regulates immune receptor signaling in hematopoietic cells and microglia, which modulates downstream cellular proliferation, survival, and chemotaxis. Here, we report the optimization and characterization of a pyridyl–pyrazole-piperidine scaffold as a basis for SHIP1 ligands that demonstrate cellular target engagement, brain exposure, and evidence of a central pharmacodynamic response in vivo. Structure–activity relationship studies guided by biochemical and cellular assays using multiple human and murine SHIP1 protein constructs and cells identified compound 32 as a SHIP1-active ligand. To ensure that the cellular activity of compound 32 is not due to kinase inhibition in general within the context of BV2 mouse microglia cells, we executed a kinome profiling study of compound 32 compared to crizotinib as a positive control using BV2 cell lysates.
HostingRepository	PRIDE
AnnounceDate	2026-04-03
AnnouncementXML	Submission_2026-04-03_09:33:26.515.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Angus
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-12 14:23:46	ID requested
⏵ 1	2026-04-03 09:33:27	announced

Dataset with its publication pending

submitter keyword: None

Timothy Richardson
contact affiliation	Indiana University School of Medicine
contact email	timorich@iu.edu
lab head
Steven Angus
contact affiliation	Indiana University School of Medicine
contact email	sangus@iu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071931
     1. Label: PRIDE project
     2. Name: Kinome profiling of BV2 cells treated with crizotinib or compound 32