PXD071927 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative TMT proteomics of myristoylated proteins in Trypanosoma cruzi and human cardiomyocytes during N-myristoyltransferase inhibition |
| Description | This project investigates the impact of N-myristoyltransferase (NMT) inhibition on the myristoylated proteome of Trypanosoma cruzi and human cardiomyocytes in the context of Chagas disease. Human AC16 cardiomyocytes were infected with T. cruzi (CL Brener strain) and treated with the reference NMT inhibitor DDD85646 or vehicle control. Myristoylated proteins from intracellular amastigotes, extracellular trypomastigotes, and host cells were metabolically labeled with an azide-functionalized myristic acid analogue, enriched by click-chemistry, digested, and labeled with TMTsixplex reagents for multiplexed quantification. Labeled peptide fractions were analyzed by nanoLC–MS/MS on a Vanquish Neo UHPLC coupled to an Orbitrap Exploris 240 operated in data-dependent acquisition mode. The resulting dataset was used to quantify stage-specific changes in parasite and host myristoylated proteins upon TcNMT inhibition and to identify pathways related to vesicular trafficking, lipid metabolism, and signal transduction that are selectively perturbed in the parasite. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-26 |
| AnnouncementXML | Submission_2026-01-25_16:52:12.319.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD071927 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Angel Torres |
| SpeciesList | scientific name: Trypanosoma cruzi; NCBI TaxID: NEWT:5693; scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | myristoylated residue |
| Instrument | Orbitrap Exploris 240 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-12 12:29:18 | ID requested | |
| ⏵ 1 | 2026-01-25 16:52:13 | announced | |
Publication List
| Gonz, á, lez Garc, í, a D, Torres A, Talevi A, Alberca LN, Beltran MA, Lara F, Da Silva Ferreira M, Farani PSG, Almeida IC, Maldonado RA, discovery and biochemical evaluation. Front Mol Biosci, 12():1666768(2025) [pubmed] |
| 10.6019/PXD071927; |
| 10.3389/fmolb.2025.1666768; |
Keyword List
| submitter keyword: N-myristoyltransferase, N-myristoylation, TMT sixplex, Orbitrap Exploris 240,Chagas disease, AC16 cardiomyocytes, Trypanosoma cruzi, click chemistry, quantitative proteomics, LC–MS/MS |
Contact List
| Dr. Rosa A. Maldonado |
|---|
| contact affiliation | Biological Sciences, The University of Texas at El Paso |
| contact email | ramaldonado@utep.edu |
| lab head | |
| Angel Torres |
|---|
| contact affiliation | The University of Texas at El Paso |
| contact email | atorres118@utep.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD071927 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071927
- Label: PRIDE project
- Name: Quantitative TMT proteomics of myristoylated proteins in Trypanosoma cruzi and human cardiomyocytes during N-myristoyltransferase inhibition
