PXD071922

PXD071922 is an original dataset announced via ProteomeXchange.

Title	The composition of reticular adhesions in RPMI-7951 and MDA-MB-435S melanoma cell lines and its comparison with the reticular adhesion composition following talin2 or KANK2 knockdown
Description	Integrins bind extracellular matrix proteins and, when clustered, form integrin adhesion complexes (IACs) that connect to and regulate the cytoskeleton, shaping normal and tumour cell behaviour. In addition to well-known nascent adhesions, focal adhesions (FAs), fibrillar adhesions (FBs), and hemidesmosomes, a newer class of IACs, reticular adhesions (RAs), has been identified. RAs, originally described as flat clathrin lattices formed by integrin αVβ5, are unique in lacking actin association and FA markers. Their roles in normal and cancer cells remain unclear. We previously showed that two melanoma cell lines, MDA-MB-435S and RPMI-7951, rely primarily on integrin αVβ5 for adhesion. Here, we provide a detailed analysis of RAs in these lines, which differ in their ability to form FBs. To define RA composition, we disrupted FAs using the actin polymerization inhibitor cytochalasin D, isolated RAs, and analysed them by MS-based proteomics. Known RA-associated proteins, including the AP-2 adaptor complex, disabled homolog 2 (DAB2) and Numb were identified in both lines, along with talin2. Notably, we also detected the presence of KN motif and ankyrin repeat domains protein (KANK2) in RA isolates. We then investigated the effect of talin2 or KANK2 knockdown on RAs composition.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_16:57:46.229.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikolina Stojanovic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-12 11:16:42	ID requested
⏵ 1	2026-06-14 16:57:46	announced

10.1186/s12964-026-02904-1;

Rac A, Lon, č, ari, ć M, Stojanovi, ć N, Fatima M, Re, š, etar M, Hr, š, ak D, Humphries JD, Humphries MJ, Ambriovi, ć, -Ristov A, Regulation of reticular adhesions by KANK2 and talin2 in two melanoma cell lines. Cell Commun Signal, 24(1):(2026) [pubmed]

submitter keyword: reticular adhesions, KANK2, matrisome, adhesome,melanoma, RPMI-7951 cells, integrin alphavbeta5, talin2, MDA-MB-435S cells

Andreja Ambriović-Ristov
contact affiliation	Laboratory for Cell Biology and Signalling, Division of Molecular Biology, Ruder Boskovic Institute, Zagreb, Croatia
contact email	Andreja.Ambriovic.Ristov@irb.hr
lab head
Nikolina Stojanovic
contact affiliation	Ruđer Bošković Institute
contact email	nikolina.stojanovic@irb.hr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD071922

PRIDE project URI

• PRIDE
  1. PXD071922
     1. Label: PRIDE project
     2. Name: The composition of reticular adhesions in RPMI-7951 and MDA-MB-435S melanoma cell lines and its comparison with the reticular adhesion composition following talin2 or KANK2 knockdown