PXD071919

PXD071919 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis identifies ATE1-dependent arginylation dysregulation across meningioma grades
Description	Meningiomas are the most common primary brain tumors, yet the molecular pathways that distinguish grade 1 from grade 2 lesions remain insufficiently understood. Among post-translational modifications, N-terminal arginylation—catalyzed by ATE1—regulates protein stability and cellular stress responses, but its role in meningioma biology has not been explored. Here, we integrated mass-spectrometry–based proteomics, immunoblotting, and transcriptomic re-analysis to investigate pathway regulation across tumor grades. Grade 1 meningiomas displayed higher ATE1 expression and increased arginylation of key chaperones, accompanied by activation of the PERK branch of the unfolded protein response (UPR), enhanced autophagy, and greater engagement of apoptosis pathways. In contrast, grade 2 tumors showed reduced ATE1 levels, diminished BIP arginylation, attenuated UPR-PERK signaling, impaired autophagy, and increased proliferative signaling. Proteins predicted to be substrates of ATE1-mediated degradation were upregulated in grade 2 tumors, suggesting that loss of arginylation may stabilize pro-oncogenic factors. Together, these findings reveal grade-specific remodeling of the N-degron/arginylation axis and highlight protein arginylation as a previously unrecognized modulator of meningioma progression with potential therapeutic relevance.
HostingRepository	PRIDE
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_01:52:06.977.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giuseppe Palmisano
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-12 09:02:58	ID requested
⏵ 1	2026-09-09 01:52:08	announced

Macedo-da-Silva J, Pereira BJA, Mule SN, Oba-Shinjo SM, Rosa-Fernandes L, Marie SKN, Palmisano G, Proteomic Analysis Identifies ATE1-Dependent Arginylation Dysregulation across Meningioma Grades. J Proteome Res, 25(8):3926-3936(2026) [pubmed]

10.1021/acs.jproteome.5c01173;

submitter keyword: meningiomas, post-translational modifications (PTMs), grade comparison, proteomics,Arginylation, ATE1

Giuseppe Palmisano
contact affiliation	Department of Parasitology, Institute of Biomedical Sciences, University of Sao Paulo
contact email	palmisano.gp@gmail.com
lab head
Giuseppe Palmisano
contact affiliation	University of Sao Paulo
contact email	palmisano.gp@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071919
     1. Label: PRIDE project
     2. Name: Proteomic analysis identifies ATE1-dependent arginylation dysregulation across meningioma grades