⮝ Full datasets listing
PXD071842
PXD071842 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of USP28/NMMHC IIA-interacting proteins in endothelial cells using immunoprecipitation-coupled mass spectrometry (IP–MS) |
| Description | To identify protein interaction partners of USP28/NMMHC IIA in endothelial cells, we performed immunoprecipitation-coupled mass spectrometry (IP–MS) using human umbilical vein endothelial cells (HUVECs). Cell lysates were incubated with anti-USP28/NMMHC IIA antibody, followed by pulldown using Protein A/G agarose beads. Eluted proteins were subjected to SDS denaturation, trypsin digestion, and liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis. MS analysis was carried out on a Thermo Scientific Q Exactive mass spectrometer coupled to an Easy-nLC system. High-resolution MS1 scans and HCD-based MS2 fragmentation enabled sensitive detection of immunoprecipitated proteins. Data-dependent acquisition (DDA) was applied to generate peptide spectra. Raw data were processed using MaxQuant for peptide identification against the UniProt database and label-free quantification. Database comparison and quantitative analysis revealed a set of specifically enriched proteins in the anti-USP28/NMMHC IIA IP samples. |
| HostingRepository | iProX |
| AnnounceDate | 2025-12-11 |
| AnnouncementXML | Submission_2025-12-10_18:13:59.440.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Junping Kou |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-10 18:13:42 | ID requested | |
| ⏵ 1 | 2025-12-10 18:14:00 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: USP28, NMMHC IIA, interacting proteins, endothelial cells, immunoprecipitation-coupled mass spectrometry |
Contact List
| Junping Kou | |
|---|---|
| contact affiliation | China Pharmaceutical University |
| contact email | junpingkou@cpu.edu.cn |
| lab head | |
| Junping Kou | |
| contact affiliation | China Pharmaceutical University |
| contact email | junpingkou@cpu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
