PXD071837

PXD071837 is an original dataset announced via ProteomeXchange.

Title	From Cell Lines To The Clinic: Identifying A Specific Urinary BlCa-EV Signature In Comparative Proteomics Of Bladder Cancer Lysates And Extracellular Vesicles
Description	Background: There is an urgent need for the development of innovative diagnostic and therapeutic strategies in bladder cancer (BlCa) management, due to its significant incidence, mortality and morbidity. Extracellular vesicles (EVs) represent highly promising candidates as biomarkers, as their cargo is highly protected from degradation and they can be isolated non-invasively from urine. Furthermore, they play a pivotal role in intercellular communication that drives disease progression, making them suitable candidates for tailored therapy approaches. Methods: We employed high-throughput mass-spectrometry to perform a comprehensive proteomic analysis of cell lysates and matched EVs. EVs were isolated from six distinct human BlCa cell lines, representatives of the complete disease spectrum, in order to identify a BlCa-EV specific signature. The translational ability of this signature was analyzed in urinary EV (uEV) samples from BlCa patients and healthy controls. Results: This study established a new lysate-to-EV signature that is common to all malignant cell lines. Afterwards, this profile was analyzed in BlCa uEVs, identifying a robust and novel 13 protein BlCa-specific signature, which includes ITGA2, ITGA6, SCRIB and TFRC. Conclusion: We unveiled a novel, specific panel of EV-derived proteins that have potential to be further applied for future functional studies, non-invasive diagnostic assays and targeted therapeutic interventions against BlCa.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_17:51:30.727.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-10 15:44:06	ID requested
⏵ 1	2026-06-07 17:51:31	announced

Louren, ç, o C, Const, â, ncio V, Tavares NT, Monteiro-Reis S, Silva-Santos R, Lobo J, Carvalho Â, Jer, ó, nimo C, From cell lines to the clinic: identifying a urinary BlCa-EV signature through comparative proteomics of bladder cancer lysates and extracellular vesicles. Cell Commun Signal, 24(1):(2026) [pubmed]

10.1186/s12964-026-02868-2;

submitter keyword: Bladder Cancer, Proteomics, Cell lines, Urine, Extracellular vesicles

Carmen Jerónimo
contact affiliation	1 - Cancer Biology & Epigenetics Group, IPO Porto Research Center (CI-IPOP), Portuguese Oncology Institute of Porto (IPO Porto), Porto, Portugal 2 - CI-IPOP @RISE-Associated Laboratory and Porto Comprehensive Cancer Center Raquel Seruca (Porto.CCC), R. Dr. António Bernardino de Almeida, Porto, Portugal
contact email	carmenjeronimo@ipoporto.min-saude.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD071837

PRIDE project URI

• PRIDE
  1. PXD071837
     1. Label: PRIDE project
     2. Name: From Cell Lines To The Clinic: Identifying A Specific Urinary BlCa-EV Signature In Comparative Proteomics Of Bladder Cancer Lysates And Extracellular Vesicles