PXD071827

PXD071827 is an original dataset announced via ProteomeXchange.

Title	Whole cell proteomics of teratoma derived from human induced pluripotent stem cells (hiPSCs) and glucosylceramide synthase-deficient (UGCG KO) hiPSCs.
Description	Glycosphingolipids (GSLs) are membrane lipids involved in numerous cellular processes and can be linked to diseases. With the aim to study the relevance of GSLs on early mammalian development, we generated glucosylceramide synthase-deficient human induced pluripotent stem cell lines (UGCG KO hiPSC). Thereby GlcCer-based GSL synthesis is completely abrogated. The UGCG KO hiPSCs maintained pluripotency as evidenced by teratoma formation in vivo. Here we compared whole cell proteomes of explanted teratoma derived from wildtype (WT) and glucosylceramide synthase-deficient (UCGG KO) hiPSCs by mass-spectrometry-based quantitative proteomics. Differential protein expressions of proteins related to pathways associated with signal transduction and protein localization were observed.
HostingRepository	PRIDE
AnnounceDate	2026-08-20
AnnouncementXML	Submission_2026-08-20_04:48:23.934.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD071827
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Julia Beimdiek
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-10 12:30:36	ID requested
⏵ 1	2026-08-20 04:48:24	announced

10.6019/PXD071827;

10.1016/J.MCPRO.2026.101630;

submitter keyword: None

Falk Büttner
contact affiliation	Proteomics, Institute of Theoretical Medicine, University of Augsburg, 86159 Augsburg, Germany
contact email	falk.buettner@med.uni-augsburg.de
lab head
Julia Beimdiek
contact affiliation	Proteomics, Institute of Theoretical Medicine, University of Augsburg, Germany
contact email	julia.beimdiek@gmx.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD071827

PRIDE project URI

• PRIDE
  1. PXD071827
     1. Label: PRIDE project
     2. Name: Whole cell proteomics of teratoma derived from human induced pluripotent stem cells (hiPSCs) and glucosylceramide synthase-deficient (UGCG KO) hiPSCs.