PXD071815

PXD071815 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomic and Functional Comparison of Extracellular Vesicles from Three GMP-Grade MSC Donors – Role of Membrane Proteins
Description	Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have emerged as promising therapeutic agents due to their anti-inflammatory and regenerative properties. Despite growing clinical interest, donor-to-donor inconsistencies remain key challenges for standardizing MSC-EV production under Good Manufacturing Practice (GMP) conditions. In this study, we aimed to systematically evaluate the molecular and functional consistency of EVs derived from three independent human adipose tissue-MSC donors. GMP-grade EVs were initially isolated using tangential flow filtration on a large scale and then characterized by multiple biophysical analyses. Quantitative proteomic profiling identified 2,615 proteins, of which 84-94% were not significantly changed across batches, highlighting a robust core proteome. Notably, 361 membrane-associated proteins were consistently conserved, including transporters, adhesion molecules, and signaling receptors, implicating these components in EV-mediated intercellular communication and immunomodulation. Functional analysis using an in vitro macrophage inflammation model demonstrated that all EV batches reproducibly suppressed pro-inflammatory cytokine production in a dose-dependent manner, with no significant inter-batch differences. Collectively, these findings indicate that MSC-EVs maintain both molecular and functional stability across different donors, and that a conserved proteomic signature underlies their reproducible anti-inflammatory activity. This study provides a foundation for establishing standardized quality criteria and advancing MSC-EVs toward clinical therapeutic applications.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:49:15.234.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Fuchs
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-10 07:51:32	ID requested
⏵ 1	2026-04-26 16:49:16	announced

10.20517/evcna.2025.173;

Park KS, Ha DH, Lee JH, Ordouzadeh N, Bergqvist M, Lee HJ, Shin E, Cho BS, L, ö, tvall J, Quantitative proteomic and functional comparison of extracellular vesicles from multiple adipose tissue mesenchymal stem cell donors. Extracell Vesicles Circ Nucl Acids, 7(1):425-440(2026) [pubmed]

submitter keyword: Mesenchymal stem cells, Membrane proteins, Therapy,Extracellular vesicles, Anti-inflammation, Consistency, Exosomes

Jan Lötvall
contact affiliation	Krefting Research Centre, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Sweden
contact email	jan.lotvall@gu.se
lab head
Johannes Fuchs
contact affiliation	Gothenburg University
contact email	johannes.fuchs@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD071815

PRIDE project URI

• PRIDE
  1. PXD071815
     1. Label: PRIDE project
     2. Name: Quantitative Proteomic and Functional Comparison of Extracellular Vesicles from Three GMP-Grade MSC Donors – Role of Membrane Proteins