PXD071809

PXD071809 is an original dataset announced via ProteomeXchange.

Title	Deficiency of OGT-mediated O-GlcNAcylation causes female infertility with oocyte meiotic arrest and follicle development defects by impairing mitochondrial function
Description	Abnormalities in oocyte meiosis are a major cause of female infertility. Since transcriptional activity is largely absent during oocyte meiosis, post-translational modifications of proteins play an essential regulatory role. Among these, OGT-mediated O-GlcNAcylation is a key modification involved in various biological processes. However, its specific function during oocyte maturation remains unclear. In this study, we demonstrate that conditional knockout of OGT in growing mouse oocytes using Gdf9-Cre leads to complete female infertility. OGT depletion prevented germinal vesicle breakdown and polar body extrusion, resulting in meiotic arrest. Although spindle formation and chromosome alignment were unaffected, OGT loss disrupted kinetochore-microtubule attachments and activated the spindle assembly checkpoint. Furthermore, OGT deletion caused excessive follicle depletion and reduced the number of ovulated oocytes. Multi-omics analysis showed that OGT-deficient oocytes exhibited downregulated expression of mitochondrial respiratory chain complex I proteins and impaired NADH-to-NAD+ conversion. Functional assays confirmed that OGT deficiency compromises mitochondrial function, as evidenced by abnormal mitochondrial distribution, decreased membrane potential, reduced ATP levels, and increased oxidative stress. In summary, our data establish OGT-mediated O-GlcNAcylation as a fundamental determinant of oocyte maturation and provide new insights into its role in supporting female fertility.
HostingRepository	PRIDE
AnnounceDate	2026-07-31
AnnouncementXML	Submission_2026-07-31_06:08:43.366.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Caiyun Wu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-10 03:26:25	ID requested
⏵ 1	2026-07-31 06:08:43	announced

10.1016/J.FREERADBIOMED.2026.02.054;

submitter keyword: OGT

O-GlcNAcylation

female infertility

oocyte

mitochondrial function

Zhiming Ding
contact affiliation	Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei 230022, China
contact email	dingzhiming10@163.com
lab head
Caiyun Wu
contact affiliation	Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University
contact email	wucy7991@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD071809

PRIDE project URI

• PRIDE
  1. PXD071809
     1. Label: PRIDE project
     2. Name: Deficiency of OGT-mediated O-GlcNAcylation causes female infertility with oocyte meiotic arrest and follicle development defects by impairing mitochondrial function