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PXD071782

PXD071782 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLabel-free quantification of MCF10A cell culture proteome (DIA) stably expressing BAD3SA in 2D vs 3D
DescriptionGlobal shotgun proteomic analysis using data independent acquisition (DIA) and Spectronaut analysis on the input cell lysates before the enrichment step in proximity labeling experiments, specifically MCF10A cells stably expressing Bcl2-associated agonist of cell death (BAD) with three serine to alanine mutations (S75A, S99A, S118A) fused with miniTurbo (miniTurbo-BAD3SA) in 2D with biotin treatment (n=3) and miniTurbo-BAD3SA in 3D with biotin treatment (n=4). The file "L20240424-miniTurbo-BAD-WT-vs-3SA-DIA.sne" contains Spectronaut search result including miniTurbo-BAD WT 2D and 3D shotgun samples found under the identifier MSV000094824 (2D) and MSV000094825 (3D).
HostingRepositoryMassIVE
AnnounceDate2025-12-10
AnnouncementXMLSubmission_2025-12-10_10:34:06.079.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOlivier Julien
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListAcetyl; Oxidation; Carbamidomethyl
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-12-09 14:45:07ID requested
12025-12-10 10:34:06announced
Publication List
no publication
Keyword List
submitter keyword: BAD, miniTurbo, BioID, LFQ, DIA, DatasetType:Proteomics
Contact List
Olivier Julien
contact affiliationUniversity of Alberta
contact emailojulien@ualberta.ca
lab head
Ing Swie Goping
contact affiliationUniversity of Alberta
contact emailigoping@ualberta.ca
lab head
Olivier Julien
contact affiliationUniversity of Alberta
contact emailojulien@ualberta.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100168/