PXD071778

PXD071778 is an original dataset announced via ProteomeXchange.

Title	Functional and protein interaction analysis of Nop7 in trypanosomatid parasites
Description	Nucleolar protein 7 (Nop7), also known as PES1 or Pescadillo, is a conserved factor essential for ribosome biogenesis, cell cycle progression, proliferation, and nucleolar organization in yeast and metazoans. Its dysregulation has been linked to several non-infectious human diseases. In this study, we characterized Nop7 in the trypanosomatids parasites Trypanosoma brucei and Leishmania major, microorganisms distinguished by their atypical ribosomes whose 28S-type ribosomal RNA is multifragmented. In silico analyses indicates that trypanosomatids Nop7 contains putative structural adaptations that could be necessary for its specialized function, working as scaffold for additional protein-protein interactions. PTP-tagged version of Nop7 localize to the nucleolus throughout cell cycle in both species. To define the Nop7 interactome, we performed tandem affinity purifications followed by mass spectrometry. This proteomic approach revealed extensive association of TbNop7 and LmNop7 with numerous transient trans-acting factors, including Erb1 and Ytm1, which together with Nop7 form a heterotrimeric subcomplex (known as the PeBow complex in mammals) required for the 60S ribosome subunit maturation in other eukaryotes. We also identified several hypothetical nucleolar proteins, representing potential novel components of the trypanosomatid ribosome biogenesis machinery. Comparative proteomic features between trypanosomatids and human underscoring parasite-specific factors potentially needed for ribosome production pathways. RNAi-mediated depletion of Nop7 in T. brucei significantly impaired proliferation, induced pronounced morphological abnormalities, and altered DNA content, highlighting its critical role in cell cycle progression and survival. Collectively, our findings provide the first proteomic characterization of Nop7-associated factors in T. brucei and L. major in culture, revealing new candidates for functional investigation and potential therapeutic targeting.
HostingRepository	PRIDE
AnnounceDate	2026-05-14
AnnouncementXML	Submission_2026-05-14_08:58:09.024.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD071778
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ebbing de Jong
SpeciesList	scientific name: Leishmania major strain Friedlin; NCBI TaxID: NEWT:347515; scientific name: Trypanosoma brucei; NCBI TaxID: NEWT:5691;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-09 13:21:37	ID requested
⏵ 1	2026-05-14 08:58:09	announced

10.6019/PXD071778;

10.1016/J.JPROT.2026.105675;

submitter keyword: Trypanosoma brucei, proteomics, Leishmania major, nucleolus, ribosome biogenesis,Nop7

Tomás Nepomuceno Mejía
contact affiliation	Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México. Av. de los Barrios 1, Col. Los Reyes Iztacala, CP 54090, Tlalnepantla de Baz, Edo. de México, México
contact email	tnepomuceno@unam.mx
lab head
Ebbing de Jong
contact affiliation	SUNY Upstate Medical University
contact email	dejonge@upstate.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD071778

PRIDE project URI

• PRIDE
  1. PXD071778
     1. Label: PRIDE project
     2. Name: Functional and protein interaction analysis of Nop7 in trypanosomatid parasites