PXD071742

PXD071742 is an original dataset announced via ProteomeXchange.

Title	Characterizing the Effects of Protein Glycosylation Perturbation on Phosphorylation Signaling
Description	This study investigates how engineered N-glycan remodeling affects phosphorylation signaling networks in human cells. We generated a glycoengineered HEK293 cell line (AI) with eliminated core fucosylation, enhanced sialylation, and reduced GlcNAc branching through sequential CRISPR/Cas9 knockouts (FUT8, MGAT4A/B, MGAT5, GMDS) and knock-in of sialuria-associated GNE mutations (R263L/R266Q), combined with stable overexpression of ST6Gal1, B4GalT1, MGAT1, and MGAT2. Both wildtype (WT) and AI cells were subjected to three treatment conditions in biological triplicate: normal growth in complete medium, 12-hour serum starvation, and 12-hour starvation followed by 30-minute serum re-stimulation (10% FBS). We performed integrated multi-omics profiling using TMT18-based quantitative proteomics to simultaneously characterize the global proteome (24 fractions), phosphoproteome (12 Fe-IMAC enriched fractions), and glycoproteome (12 MAX-enriched fractions) across all conditions. This dataset enables systematic interrogation of glycan-phosphorylation crosstalk and identifies glycan-dependent signaling rewiring in response to growth factor stimulation.
HostingRepository	PRIDE
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_05:54:06.425.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongyi Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	N-glycosylated residue; phosphorylated residue
Instrument	timsTOF HT; Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-08 19:37:14	ID requested
⏵ 1	2026-09-09 05:54:07	announced

Wei E, Liu H, Betenbaugh M, Zhang H, Characterizing the Effects of Protein Glycosylation Perturbation on Phosphorylation Signaling. Anal Chem, 98(25):18577-18595(2026) [pubmed]

10.1021/acs.analchem.6c00790;

submitter keyword: proteomics,glycosylation, phosphorylation, serum starvation, serum stimulation, glycoengineering

Hui Zhang
contact affiliation	Johns Hopkins University
contact email	huizhang@jhu.edu
lab head
Hongyi Liu
contact affiliation	Johns Hopkins University
contact email	hliu173@jh.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071742
     1. Label: PRIDE project
     2. Name: Characterizing the Effects of Protein Glycosylation Perturbation on Phosphorylation Signaling