PXD071724 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mapping ISG15 sites on GAPDH and PGK1 by AP-MS |
| Description | We employed an AP-MS approach to map ISG15 modification sites on GAPDH and PGK1 in HEK293T cells by overexpression of FLAG-GAPDH, FLAG-PGK1 or FLAG-GFP together with the ISG15 conjugation enzymes (E1, E2, E3) and wild-type HA-ISG15 or conjugation-defective ISG15-AA. Cells were lysed in physiological buffer, subjected to FLAG-pulldown followed by on-bead digestion and further processing for LC-MS/MS analysis. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-02 |
| AnnouncementXML | Submission_2026-03-02_10:34:01.647.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Denzel Eggermont |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | ubiquitination signature dipeptidyl lysine; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-08 11:22:43 | ID requested | |
| ⏵ 1 | 2026-03-02 10:34:02 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: AP-MS, PGK1, GAPDH,ISG15, glycolysis |
Contact List
| Francis Impens |
|---|
| contact affiliation | VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium |
| contact email | francis.impens@vib-ugent.be |
| lab head | |
| Denzel Eggermont |
|---|
| contact affiliation | VIB-Ugent Center for Medical Biotechnology |
| contact email | denzel.eggermont@vib-ugent.be |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD071724 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071724
- Label: PRIDE project
- Name: Mapping ISG15 sites on GAPDH and PGK1 by AP-MS
