PXD071678

PXD071678 is an original dataset announced via ProteomeXchange.

Title	Evolutionarily conserved spliceosome-exosome pathway in nuclear mRNA surveillance
Description	Intron-containing mRNAs are co-transcriptionally spliced and assembled into messenger ribonucleoprotein (mRNP) particles, a process monitored by surveillance pathways. Here, we combined biochemical and structural approaches to elucidate the mechanisms by which mRNPs are sorted between two opposing fates: nuclear degradation or cytoplasmic export. The human LENG8-PCID2 complex operates as an mRNP-decay connector, coupling nuclear mRNPs to the RNA-degrading exosome via the PAXT adaptor complex, while the related GANP-PCID2 complex connects mRNPs to the nuclear pore complex. Both recognize the mRNP component UAP56, but LENG8-PCID2 uniquely associates with early splicing factors through a direct interaction with U1A and RRP1B. Notably, the Thp3-Csn12 ortholog in budding yeast also serves as an mRNP-decay connector, coupling the early splicing factors Mud2-Bbp with the nuclear exosome. The spliceosome-exosome mRNP-decay pathway we uncovered reveals molecular principles that remain strikingly conserved across evolution, despite the fundamental differences in splicing and decay between humans and budding yeast.
HostingRepository	PRIDE
AnnounceDate	2026-05-18
AnnouncementXML	Submission_2026-05-18_07:00:52.730.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Steigenberger
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-07 10:57:50	ID requested
⏵ 1	2026-05-18 07:00:53	announced

10.1101/GAD.353594.125;

submitter keyword: UAP56, cryo-EM, RRP1B, TREX-2, missplicing,Exosome-mediated decay, quality control, mRNA export, BioID

Elena Conti
contact affiliation	Department of Structural Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried, 82152 Munich, Germany.
contact email	conti@biochem.mpg.de
lab head
Barbara Steigenberger
contact affiliation	MPI of Biochemistry
contact email	steigenberger@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD071678

PRIDE project URI

• PRIDE
  1. PXD071678
     1. Label: PRIDE project
     2. Name: Evolutionarily conserved spliceosome-exosome pathway in nuclear mRNA surveillance