PXD071677

PXD071677 is an original dataset announced via ProteomeXchange.

Title	Identification of ADAR2-associated complexes
Description	Adenosine deaminase acting on RNA 2 (ADAR2) catalyzes adenosine-to-inosine RNA editing and contributes to multiple aspects of post-transcriptional regulation. Proximity-labeling (BioID) studies have previously defined the proximal protein environment of ADAR2. To complement these datasets and identify stably associated proteins, we performed Strep-tag affinity purification coupled to label-free LC–MS/MS. Doxycycline-inducible 293 T-REx cells expressing N-terminally Strep II–HA–tagged ADAR2 were used to isolate ADAR2-containing complexes under native conditions, followed by high-resolution mass spectrometric identification and quantification of co-purifying proteins. The resulting ADAR2 interactome shows significant overlap with our previously defined ADAR1 interaction network, as well as with publicly available ADAR2 proximity-labeling datasets, supporting the robustness and reproducibility of ADAR-associated protein complexes across methods and studies. This dataset provides a focused map of the stable ADAR2 interactome. This proteomic data form the basis of the first figure in the associated ADAR–exosome manuscript and complement existing proximity-labeling data by defining stable ADAR2 protein complexes.
HostingRepository	PRIDE
AnnounceDate	2026-04-30
AnnouncementXML	Submission_2026-04-30_06:51:55.310.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dragana Vukic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-07 09:47:41	ID requested
⏵ 1	2026-04-30 06:51:55	announced

10.1093/NAR/GKAG381;

submitter keyword: Human, HEK Flp-In 293 T-REx™ cell, affinity Purification, ADAR2 WT-HA-StrepII

Prof. Mary Anne O´Connell, Ph.D.
contact affiliation	CEITEC, Masaryk University Molecular medicine department, RNA and immunity group
contact email	mary.oconnell@ceitec.muni.cz
lab head
Dragana Vukic
contact affiliation	Central European Institute for Technology at Masaryk University
contact email	dragana.vukic@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD071677

PRIDE project URI

• PRIDE
  1. PXD071677
     1. Label: PRIDE project
     2. Name: Identification of ADAR2-associated complexes