⮝ Full datasets listing
PXD071647
PXD071647 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics reveals extensive phosphoregulation of outer kinetochore protein KNL1 |
| Description | The goal of this project was to utilize a proteomics approach to identify phosphorylation sites on native KNL1 purified from HEK293T/17 cells. KNL1 phosphorylation is known to be critical for activation of the spindle assembly checkpoint and recruitment of the fibrous corona in the presence of unattached kinetochores. We tested KNL1 phosphorylation in 4 different kinetochore-microtubule attachment states to identify additional sites that may be involved in regulating kinetochore function. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-12 |
| AnnouncementXML | Submission_2026-03-12_11:45:28.469.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Fred Hutch Proteomics Core |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Phospho |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-05 10:42:00 | ID requested | |
| ⏵ 1 | 2026-03-12 11:45:29 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Kinetchore, LC-MS/MS, anti-FLAG immunoprecipitation, DatasetType:Proteomics |
Contact List
| Susan Biggins | |
|---|---|
| contact affiliation | Fred Hutchinson Cancer Center |
| contact email | sbiggins@fredhutch.org |
| lab head | |
| Fred Hutch Proteomics Core | |
| contact affiliation | Fred Hutchinson Cancer Research Center |
| contact email | pgafken@fredhutch.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100123/ |
