PXD071603

PXD071603 is an original dataset announced via ProteomeXchange.

Title	Dysregulation of Non-Muscle Myosin IIA Assembly and Phosphorylation in S100A4 Null Mouse Lens
Description	Non-muscle myosin IIA (NMIIA), a motor protein plays a critical role in regulating cell morphology, adhesion, migration, contractility, and mechanotransduction across various tissues, including the ocular lens. S100A4, a known NMIIA-interacting protein, is abundantly expressed in lens fibers and shows discrete spatial distribution. Loss of S100A4 has previously been shown to associate with late-onset lens opacification in mice. However, its role in regulating NMIIA activity, assembly, and actin cytoskeletal organization in the lens remains unclear. Using S100A4-null mice, this study reveals that S100A4 co-immunoprecipitates with NMIIA and that its absence leads to decreased NMIIA (Ser1943) phosphorylation, impaired NMIIA filament assembly, and disruption in actin cytoskeletal organization and polymerization in lens. Quantitative proteomic analysis of cytoskeletal- and membrane-enriched fractions from S100A4-null versus wild-type lenses further identified decreased levels of CLIC5 and RNA binding protein-SERBP1. Moreover, treatment of wild-type mouse lenses in ex-vivo with trifluoperazine, a known S100A4 inhibitor, induced lens opacification in association with increased insolubilization of S100A4, NMIIA and actin. Collectively, these findings demonstrate that S100A4 plays a critical role in regulating NMIIA activity and assembly and maintaining actin cytoskeletal organization in the ocular lens, thereby contributing to lens transparency and homeostasis.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:36:48.497.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD071603
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nik Skiba
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-04 18:38:42	ID requested
⏵ 1	2026-06-28 16:36:49	announced

10.6019/PXD071603;

10.1002/jcp.70202;

Maddala R, Lankford LK, Skiba NP, Rao PV, Dysregulation of Non-Muscle Myosin IIA Assembly and Phosphorylation in S100A4 Null Mouse Lens. J Cell Physiol, 241(6):e70202(2026) [pubmed]

submitter keyword: mouse, actin cytoskeleton, cataract, S1004A, Myosin IIA, lens

Ponugoti V. Rao
contact affiliation	Duke University Eye Center
contact email	p.rao@duke.edu
lab head
Nik Skiba
contact affiliation	Duke University
contact email	skibanik@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071603
     1. Label: PRIDE project
     2. Name: Dysregulation of Non-Muscle Myosin IIA Assembly and Phosphorylation in S100A4 Null Mouse Lens