PXD071577 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Evaluation of N8-propargyl spermidine and spermine as substrates of deoxyhypusine synthase in the in vitro hypusination of eIF5A and RIPK1 |
| Description | eIF5A is a well-established protein substrate of deoxyhypusine synthase (DHS), which also requires spermidine as a small-molecule substrate. At the end of 2024, a publication claimed that RIPK1 is an additional DHS substrate and that N8-propargyl spermidine can also serve as a DHS substrate. To evaluate these claims, eIF5A and RIPK1 were subjected to in vitro hypusination in the presence of DHS, deoxyhypusine hydroxylase (DOHH; the enzyme that hydroxylates the deoxyhypusine residue generated by DHS), and either N8-propargyl spermidine or spermidine. For reactions containing N8-propargyl spermidine, the samples were split: one portion was directly digested with trypsin, whereas the other was subjected to CuAAC ligation with azido-PEG3-biotin prior to tryptic digestion. Following LC-MS/MS analysis and MaxQuant processing, the data were searched for peptides bearing hypusine, deoxyhypusine, and their N-propargylated analogs. In samples that underwent CuAAC ligation, additional searches were performed for PEG3-biotin-triazole-modified N8-propargyl-spermidine-derived hypusine and deoxyhypusine, as well as for cysteine-N8-propargyl spermidine thio-triazole adducts. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-13 |
| AnnouncementXML | Submission_2026-03-13_03:35:24.711.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Remigiusz Serwa |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | L-deoxyhypusine; hypusine; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-04 05:26:16 | ID requested | |
| ⏵ 1 | 2026-03-13 03:35:25 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: N8-propargyl spermidine, DHS, eIF5A, chemical probe, hypusination, deoxyhypusine synthase, RIPK1 |
Contact List
| Remigiusz Serwa |
|---|
| contact affiliation | IMol, Polish Academy of Sciences, M. Flisa 6, 02-247 Warsaw, Poland |
| contact email | r.serwa@imol.edu.pl |
| lab head | |
| Remigiusz Serwa |
|---|
| contact affiliation | The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences |
| contact email | r.serwa@imol.edu.pl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071577
- Label: PRIDE project
- Name: Evaluation of N8-propargyl spermidine and spermine as substrates of deoxyhypusine synthase in the in vitro hypusination of eIF5A and RIPK1
