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PXD071533
PXD071533 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The degradable kinome of DCAF1-targeting PROTAC |
| Description | Proteolysis targeting chimeras (PROTACs) have emerged as novel drug modalities, but the development of PROTACs currently relies on a limited number of E3 ligase ligands, primarily targeting CRBN and VHL. Validating novel E3 ligase ligands could expand the degradable target space. However, validation data on these understudied degrader systems are typically limited to a few highly degradable targets, such as BRD4. In the present study, we used our previously established workflow for E3 ligase ligand validation, employing promiscuous kinase PROTACs, to evaluate the potential of targeting DCAF1 for PROTAC development. Due to their similar architecture, we compared the degradable kinome of DCAF1 with an analogous set of CRBN-targeting PROTACs. Our study revealed DCAF1-dependent degradation of a diverse set of kinases, which were validated in orthogonal assays. We found a significant overlap of the degradable kinome with CRBN recruiting PROTACs, suggesting an alternative design strategy using available structurally diverse DCAF1 ligands. Furthermore, a comparative analysis of DCAF1 and CRBN PROTAC-induced ubiquitination patterns provided insight into substrate selectivity and isoform-selective degradation. The presented data will establish DCAF1-targeting PROTACs as a versatile design strategy for future degrader development. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-15 |
| AnnouncementXML | Submission_2026-06-15_04:33:47.356.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Uli Ohmayer |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | GG |
| Instrument | timsTOF Pro 2; timsTOF HT; timsTOF Ultra 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-03 07:21:32 | ID requested | |
| ⏵ 1 | 2026-06-15 04:33:47 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: DCAF1, PROTAC, Targeted Protein degradation, CRBN, Proteomics, Ubiquitinomics, K-GG remnant profiling, DatasetType:Proteomics |
Contact List
| Stefan Knapp | |
|---|---|
| contact affiliation | Institute for Pharmaceutical Chemistry, Department of Biochemistry, Chemistry and Pharmacy, Goethe University Frankfurt |
| contact email | Knapp@pharmchem.uni-frankfurt.de |
| lab head | |
| Uli Ohmayer | |
| contact affiliation | NEOsphere Biotechnologies GmbH |
| contact email | uli.ohmayer@neospherebiotech.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100084/ |
