PXD071481

PXD071481 is an original dataset announced via ProteomeXchange.

Title	Secretome proteomics of THP-1 macrophages under M0, M1, and M2 polarization
Description	This dataset comprises label-free quantitative proteomics of the secretome from human THP-1 serglycin (SRGN) knock-out and wild-type macrophage-like cells differentiated with PMA (M0) and polarized to M1 (LPS + IFN-γ) or M2 (IL-4 + IL-13). The study investigates how macrophage activation states in the absence of serglycin reshape the composition of secreted proteins relevant to inflammatory signaling, vesicle biology, and metabolism. Conditioned media were collected after polarization, processed by bottom-up proteomics, and analyzed by nanoLC–MS/MS on an Orbitrap Fusion Lumos. The resulting protein abundance profiles provide a resource to compare resting (M0) versus pro-inflammatory (M1) and alternatively activated (M2) macrophage secretomes in a standardized cell system.
HostingRepository	PRIDE
AnnounceDate	2026-02-25
AnnouncementXML	Submission_2026-02-25_07:07:34.008.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Erik Knutsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-02 10:30:35	ID requested
⏵ 1	2026-02-25 07:07:34	announced

Dataset with its publication pending

submitter keyword: SRGN knockout, macrophages,Homo sapiens, MaxQuant, label-free quantification, Orbitrap Fusion Lumos, THP-1, nanoLC-MS/MS, secretome

Erik Knutsen
contact affiliation	Tumor Biology Research Group, Department of Medical Biology, Faculty of Health Sciences, UiT The Arctic University of Norway, Tromsø, Norway
contact email	erik.knutsen@uit.no
lab head
Erik Knutsen
contact affiliation	UiT The Arctic University of Norway
contact email	erik.knutsen@uit.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD071481

PRIDE project URI

• PRIDE
  1. PXD071481
     1. Label: PRIDE project
     2. Name: Secretome proteomics of THP-1 macrophages under M0, M1, and M2 polarization