PXD071477 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Evaluation of N8-propargyl spermidine as a substrate of deoxyhypusine synthase in cellulo |
| Description | Two complementary experiments were performed to evaluate whether N8-propargyl spermidine is a substrate of deoxyhypusine synthase in cells. HEK293 and mouse embryonic fibroblast (MEF) cells were incubated with either N8-propargyl spermidine or spermidine, lysed, and subjected to CuAAC ligation with azido-PEG3-biotin. In the MEF experiment, biotinylated proteins were enriched on NeutrAvidin agarose resin, digested on-bead with trypsin, and, following TMT labeling and LC-MS/MS analysis, the data were processed in MaxQuant and analyzed for intensity enrichment in samples derived from cells treated with N8-propargyl spermidine versus spermidine. In the HEK293 experiment, proteins were digested with trypsin, biotinylated peptides were enriched on NeutrAvidin agarose resin, and after LC-MS/MS analysis and MaxQuant processing, the peptides were examined for the presence of N8-propargyl spermidine thio-triazole adducts on cysteine residues. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-13 |
| AnnouncementXML | Submission_2026-03-13_03:29:31.512.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Remigiusz Serwa |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-12-02 09:24:18 | ID requested | |
| ⏵ 1 | 2026-03-13 03:29:32 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: chemical probe, hypusination, HEK293, N8-propargyl spermidine,MEF |
Contact List
| Remigiusz Serwa |
|---|
| contact affiliation | IMol, Polish Academy of Sciences, M. Flisa 6, 02-247 Warsaw, Poland |
| contact email | r.serwa@imol.edu.pl |
| lab head | |
| Remigiusz Serwa |
|---|
| contact affiliation | The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences |
| contact email | r.serwa@imol.edu.pl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD071477 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD071477
- Label: PRIDE project
- Name: Evaluation of N8-propargyl spermidine as a substrate of deoxyhypusine synthase in cellulo
