⮝ Full datasets listing
PXD071463
PXD071463 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Regulation of HSP90AA1/NRF2 by BDNF Contributes to the Attenuation of Rotenone-Induced Oxidative Stress in Parkinson's Disease Models |
| Description | Parkinson's disease is a neurodegenerative condition characterized by dopaminergic neuron degeneration and α-synuclein accumulation, both exacerbated by oxidative stress. This study employed rotenone-treated SH-SY5Y cells to evaluate cell viability, ROS levels, and mitochondrial function. RNA-seq, mass spectrometry, and Co-IP identified BDNF-regulated proteins associated with oxidative stress. In rotenone-induced PD mice, assessments focused on motor performance, neuronal loss, and protein expression. Results demonstrated that exposure to 0.5 μM rotenone for 36 hours elevated ROS production, mitochondrial dysfunction, and cellular damage in SH-SY5Y cells, effects reversed by BDNF overexpression. In mice, BDNF overexpression in the substantia nigra pars compacta alleviated PD-like symptoms. Co-IP analysis revealed that BDNF modulates NRF2 and its associated proteins through HSP90AA1. Collectively, BDNF mitigates rotenone-induced oxidative stress in PD models via the HSP90AA1/NRF2 pathway, providing valuable insights into the pathogenesis and potential treatment of Parkinson's disease. |
| HostingRepository | iProX |
| AnnounceDate | 2025-12-02 |
| AnnouncementXML | Submission_2025-12-02_01:23:20.780.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhao Wang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-02 01:22:56 | ID requested | |
| ⏵ 1 | 2025-12-02 01:23:21 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: LC-MS/MS |
Contact List
| Zhao Wang | |
|---|---|
| contact affiliation | Department of Geriatric Medicine, Shanxi Bethune Hospital, Shanxi Academy of Medical Science |
| contact email | wangzhaodoctor1988@163.com |
| lab head | |
| Zhao Wang | |
| contact affiliation | Department of Geriatric Medicine, Shanxi Bethune Hospital, Shanxi Academy of Medical Science |
| contact email | wangzhaodoctor1988@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
