PXD071454

PXD071454 is an original dataset announced via ProteomeXchange.

Title	Characterization of secreted proteins by symbiont technology
Description	By adding Agrobacterium plant growth regulator genes to a binary plant transformation vector also encoding a gene of interest, we engineered plant tissues capable of autonomous cell division and vascularization while expressing biomolecules on the stems of host plants and in vitro plant cell tissue culture cured of Agrobacterium. We refer to these repurposed Agrobacterium galls as symbionts to reflect their intended beneficial interaction with the host plant.  When grown in vitro, symbionts efficiently export secreted proteins into the culture media and may serve as an advantageous platform for heterologous protein expression from plant cells.  In this experiment we monitored accumulation of a secreted marker portein (Flowering Locus T, tagged with mCherry) in the media of tomato cell symbionts grown in-vitro and characterized the native proteins that also accumulated in the culture medium.
HostingRepository	PRIDE
AnnounceDate	2026-03-06
AnnouncementXML	Submission_2026-03-06_06:46:40.114.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD071454
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Samuel Coradetti
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: NEWT:4081;
ModificationList	amidated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-01 22:00:22	ID requested
⏵ 1	2026-03-06 06:46:40	announced

10.3389/FPLS.2026.1754357;

10.6019/PXD071454;

submitter keyword: In-vitro protein production, cell culture,Symbiont

Michelle Heck
contact affiliation	USDA Agricultural Research Service
contact email	michelle.heck@usda.gov
lab head
Samuel Coradetti
contact affiliation	Boyce Thompson Institute
contact email	stc85@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD071454

PRIDE project URI

• PRIDE
  1. PXD071454
     1. Label: PRIDE project
     2. Name: Characterization of secreted proteins by symbiont technology