PXD071439

PXD071439 is an original dataset announced via ProteomeXchange.

Title	Red bone marrow hosts metabolically active enucleated adipocytes that support hematopoiesis
Description	Bone marrow adipocytes (BMAds) are a major component of the bone marrow (BM) that regulates bone turnover and hematopoiesis. In rodents, two distinct adipocyte populations exist: constitutive BMAds (cBMAds), located in areas devoid of hematopoietic cells and resistant to metabolic cues, and regulatory BMAds (rBMAds), interspersed within hematopoietic niches and responsive to energy stress. Despite their potential importance, rBMAds have remained poorly characterized due to their scarcity in rodents. Here, we used a high-yield method to isolate human rBMAds, enabling structural, proteomic, lipidomic, and functional analyses. Remarkably, human rBMAds are anucleate yet retain organelles and sustain lipid and glucose metabolic pathways but, unlike rodent rBMAds, display no lipolytic activity. They actively secrete factors that support hematopoiesis in vitro, implicating them as functional contributors to the BM niche. These findings suggest that human rBMAds may arise from cBMAds through terminal differentiation and define a novel adipocyte subtype critical for BM homeostasis.
HostingRepository	PRIDE
AnnounceDate	2026-06-01
AnnouncementXML	Submission_2026-06-01_00:45:29.149.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmanuelle Mouton Barbosa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-01 11:17:40	ID requested
⏵ 1	2026-06-01 00:45:29	announced

Dataset with its publication pending

submitter keyword: metabolism, adipokines, enucleated adipocytes, hematopoiesis,Bone marrow adipocytes

Odile Burlet-Schiltz
contact affiliation	1 Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, Toulouse, France. Equipe labellisée par la Ligue contre le cancer 2 Infrastructure Nationale de Protéomique, ProFI, FR 2048, Toulouse, France.
contact email	schiltz@ipbs.fr
lab head
Emmanuelle Mouton Barbosa
contact affiliation	IPBS CNRS
contact email	emmanuelle.mouton@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD071439

PRIDE project URI

• PRIDE
  1. PXD071439
     1. Label: PRIDE project
     2. Name: Red bone marrow hosts metabolically active enucleated adipocytes that support hematopoiesis