⮝ Full datasets listing
PXD071428
PXD071428 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | LCMS Analysis of V5-KEAP1 +/- Drug, Wood Lab |
| Description | Coomassie stained SDS-PAGE bands were subjected to standardized in-gel trypsin digestion in which gel bands were subjected to reduction with 10 mM dithiolthreitol, alkylated with 20 mM iodoacetamide, and digested with 100ng of sequencing grade modified trypsin (Promega). Extracted peptides were lyophilized to dryness and resuspended in 12 uL of 0.2% formic acid/2% acetonitrile. Samples were subjected to chromatographic separation on a Waters NanoAquity UPLC equipped with a 1.7 um BEH130 C18 75 um I.D. x 250 mm reversed-phase column. The mobile phase consisted of (A) 0.1% formic acid in water and (B) 0.1% formic acid in acetonitrile. Following a 3 uL injection, peptides were trapped for 3 min on a 5 um Symmetry C18 180 um I.D. x 20 mm column at 5 ul/min in 99.9% A. The analytical column was then switched in-line and a linear elution gradient of 5% B to 40% B was performed over 30 min at 400 nL/min. The analytical column was connected to a fused silica PicoTip emitter (New Objective, Cambridge, MA) with a 10 um tip orifice and coupled to an Oribtrap Fusion Lumos mass spectrometer (Thermo) through an electrospray interface operating in a data-dependent mode of acquisition. The instrument was set to acquire a precursor MS scan from m/z 200-1500 in the Orbitrap at r=120,000 with MS/MS spectra acquired in the Ion Trap with AGC setting of 1e4 and 100ms. For all experiments, stepped HCD energy settings were 28.5, 30, 31.5v and a 20 s dynamic exclusion was employed for previously fragmented precursor ions. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-12-07 |
| AnnouncementXML | Submission_2025-12-07_13:02:17.277.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Erik Soderblom |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-12-01 06:09:34 | ID requested | |
| ⏵ 1 | 2025-12-07 13:02:17 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: lcms, keap1, DatasetType:Proteomics |
Contact List
| Kris Wood | |
|---|---|
| contact affiliation | Duke University |
| contact email | kris.wood@duke.edu |
| lab head | |
| Erik Soderblom | |
| contact affiliation | Duke University |
| contact email | es114@duke.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100060/ |
