PXD071392

PXD071392 is an original dataset announced via ProteomeXchange.

Title	The mass spectrometry analysis of CTDP1 function
Description	We previously identified an interaction between the RNA polymerase II (Pol II) subunit RPB7 and the phosphatase CTDP1 in regulating transcription termination-reinitiation. Here, we establish that CTDP1 dephosphorylates a unique Pol II phospho-isoform modified at Tyr1, Ser5, and Ser7 of its C-terminal domain. This activity is critical for recruiting the Mediator complex and modulating transcriptional efficiency. Furthermore, by integrating phosphoproteomic and immunoprecipitation-mass spectrometry data, we reveal that CTDP1 also governs the phosphorylation of numerous RNA splicing factors. Our findings position CTDP1 as a master coordinator that integrates transcription with RNA splicing.
HostingRepository	PRIDE
AnnounceDate	2026-07-10
AnnouncementXML	Submission_2026-07-10_00:29:51.809.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haonan Zheng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-30 01:37:32	ID requested
⏵ 1	2026-07-10 00:29:52	announced

Dataset with its publication pending

submitter keyword: Pol II, Mediator, CTDP1

Xiong Ji
contact affiliation	Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
contact email	xiongji@pku.edu.cn
lab head
Haonan Zheng
contact affiliation	Peking University
contact email	zhenghn@pku.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071392
     1. Label: PRIDE project
     2. Name: The mass spectrometry analysis of CTDP1 function