PXD071373

PXD071373 is an original dataset announced via ProteomeXchange.

Title	Chromatin-associated protein networks of CEBPA and SUMO2/3 identified with RIME
Description	We identified the chromatin-associated protein networks of CEBPA and SUMO2/3 in BLaER1 cells during the early stages of B-cell-to-macrophage transdifferentiation. Additionally, we studied the impact of SUMOylation inhibition (SUMOi) on CEBPA protein network composition utilizing the small molecule inhibitor ML-792. Protein network members were identified with Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME) at 0, 6 and 24 h after induction of transdifferentiation in the absence and presence of ML-792. Mass spectrometry analyses were executed with high resolution mass spectrometry (LC-MS), using the Evosep One liquid chromatography system coupled to a hybrid trapped ion mobility quadrupole TOF mass spectrometer (Bruker timsTOF Pro) via a CaptiveSpray nano-electrospray ion source. High-confidence chromatome members were discriminated from background with SAINT analysis.
HostingRepository	PRIDE
AnnounceDate	2026-05-27
AnnouncementXML	Submission_2026-05-27_08:56:20.530.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emma Valima
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-28 10:14:17	ID requested
⏵ 1	2026-05-27 08:56:21	announced

10.1007/S00018-026-06255-5;

submitter keyword: mass spectrometry, transdifferentiation, immunoprecipitation,human, chromatin

Jorma J. Palvimo
contact affiliation	Institute of Biomedicine, School of Medicine, University of Eastern Finland, Finland
contact email	jorma.palvimo@uef.fi
lab head
Emma Valima
contact affiliation	Institute of Biomedicine, School of Medicine, University of Eastern Finland
contact email	emma.valima@uef.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD071373
     1. Label: PRIDE project
     2. Name: Chromatin-associated protein networks of CEBPA and SUMO2/3 identified with RIME